Department of Respiratory and Critical Care Medicine, Renji Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
Immunol Res. 2024 Oct;72(5):1174-1184. doi: 10.1007/s12026-024-09520-6. Epub 2024 Jul 30.
Our previous bioinformatics analysis has revealed that Rab-interacting lysosomal protein-like 2 (RILPL2) is associated with tumor immune microenvironment in non-small cell lung cancer (NSCLC). In our study, we collected 140 patients with primary NSCLC to verify the RILPL2 expression and its prognostic value, the relationship between RILPL2 expression and CD4, CD8T cell infiltration. A total of 140 patients who had been diagnosed with primary NSCLC (including 66 lung adenocarcinomas and 74 lung squamous cell carcinomas) were enrolled in our study. Immunohistochemical (IHC) staining was performed to analyze the expression of RILPL2, CD4, and CD8 in these patients. Compared with peri-cancer tissues, the RILPL2 expression in NSCLC tissues was significantly lower (P < 0.0001). RILPL2 expression was significantly related to clinical stage (P = 0.019), and low RILPL2 expression indicated higher stage. Low RILPL2 expression predicted worse overall survival (OS) in NSCLC patients (P = 0.017). Correlational analyses revealed that RILPL2 expression was significantly positively correlated with CD4T cell infiltration in NSCLC (R = 0.294, P < 0.001), LUAD subgroup (R = 0.256, P = 0.038), and LUSC subgroup (R = 0.333, P = 0.004); RILPL2 expression was also significantly positively correlated with CD8 T cell infiltration in NSCLC (R = 0.263, P = 0.002), LUAD subgroup (R = 0.280, P = 0.023), and LUSC subgroup (R = 0.250, P = 0.031). In conclusion, RILPL2 expression was downregulated in NSCLC; low RILPL2 expression was significantly related to higher stage and worse prognosis; RILPL2 expression was significantly positively correlated with CD4, CD8T cell infiltration.
我们之前的生物信息学分析表明，Rab 相互作用的溶酶体蛋白样 2（RILPL2）与非小细胞肺癌（NSCLC）的肿瘤免疫微环境有关。在我们的研究中，我们收集了 140 名原发性 NSCLC 患者的样本，以验证 RILPL2 的表达及其预后价值，以及 RILPL2 表达与 CD4、CD8T 细胞浸润的关系。共纳入 140 例初诊 NSCLC 患者（包括 66 例肺腺癌和 74 例肺鳞癌），采用免疫组化（IHC）染色分析 RILPL2、CD4 和 CD8 在这些患者中的表达。与癌旁组织相比，NSCLC 组织中 RILPL2 的表达明显降低（P<0.0001）。RILPL2 表达与临床分期显著相关（P=0.019），低 RILPL2 表达提示分期较高。低 RILPL2 表达预示 NSCLC 患者总生存期（OS）较差（P=0.017）。相关性分析显示，RILPL2 表达与 NSCLC 中 CD4T 细胞浸润呈显著正相关（R=0.294，P<0.001），LUAD 亚组（R=0.256，P=0.038）和 LUSC 亚组（R=0.333，P=0.004）；RILPL2 表达与 NSCLC 中 CD8T 细胞浸润也呈显著正相关（R=0.263，P=0.002），LUAD 亚组（R=0.280，P=0.023）和 LUSC 亚组（R=0.250，P=0.031）。综上所述，RILPL2 在 NSCLC 中表达下调；低 RILPL2 表达与较高的分期和较差的预后显著相关；RILPL2 表达与 CD4、CD8T 细胞浸润呈显著正相关。
