Heyman B, Hobbs M V, Weigle W O
Cell Immunol. 1985 Apr 15;92(1):134-41. doi: 10.1016/0008-8749(85)90071-1.
The direct splenic anti-sheep erythrocyte (anti-SRBC) responses as well as the serum IgG1, IgG2a, IgG2b, and IgG3 anti-SRBC responses of CBA/CaJ mice were monitored 4-35 days after immunization with: (1) a suboptimal dose of SRBC, (2) a suboptimal dose of SRBC plus monoclonal IgM anti-SRBC, or (3) a high dose of SRBC. The direct plaque-forming cell (PFC) responses of mice in treatment group 2 were significantly higher than those in group 1 but similar to the responses in group 3. The serum anti-SRBC antibody responses of all IgG subclasses were significantly enhanced by IgM anti-SRBC and were generally even higher than the responses obtained with high doses of SRBC. The relative proportions of each serum IgG subclass were similar in all three groups. These data suggest that the enhancement of suboptimal anti-SRBC antibody responses by IgM anti-SRBC extends through IgM and all of the IgG subclasses and, further, that the isotype profile in antibody-enhanced responses is similar to that obtained with high doses of SRBC.
在使用以下物质免疫CBA/CaJ小鼠后4至35天，监测其脾脏对绵羊红细胞（抗SRBC）的直接反应以及血清IgG1、IgG2a、IgG2b和IgG3抗SRBC反应：（1）次优剂量的SRBC，（2）次优剂量的SRBC加单克隆IgM抗SRBC，或（3）高剂量的SRBC。治疗组2小鼠的直接空斑形成细胞（PFC）反应显著高于组1，但与组3的反应相似。所有IgG亚类的血清抗SRBC抗体反应均被IgM抗SRBC显著增强，且总体上甚至高于高剂量SRBC所获得的反应。三组中各血清IgG亚类的相对比例相似。这些数据表明，IgM抗SRBC对次优抗SRBC抗体反应的增强作用贯穿IgM和所有IgG亚类，此外，抗体增强反应中的同种型谱与高剂量SRBC所获得的相似。
