Choromanski L, Kuhn R E
Immunology. 1986 Oct;59(2):289-94.
Mice infected with Trypanosoma cruzi, and profoundly suppressed in their ability to respond to sheep erythrocytes (SRBC), were found to become highly responsive to these antigens when given two injections of SRBC at 4- or 6-day intervals. Two injections at intervals of 2, 8 or 10 days did not restore responsiveness. The ability to overcome immunosuppression via two challenges with antigen was found to be antigen-specific, in that if the first injection was with SRBC and the second with horse RBC there was no enhancement of plaque-forming cells to either antigen. Homologous challenges with SRBC or HRBC, however, did overcome immunosuppression. It is suggested that the ability to overcome immunosuppression by two injections of antigens at 4- or 6-day intervals is due to stimulation of a small number of T-helper cells in the first injection and an expansion of these cells in the second injection resulting in sufficient help to induce specific B-cell responses.
感染克氏锥虫且对绵羊红细胞（SRBC）反应能力严重受抑的小鼠，在每隔4天或6天注射两次SRBC后，对这些抗原的反应变得高度敏感。每隔2天、8天或10天注射两次则无法恢复反应能力。通过两次抗原激发克服免疫抑制的能力具有抗原特异性，即如果第一次注射的是SRBC，第二次注射的是马红细胞，那么对任何一种抗原的噬斑形成细胞都不会增加。然而，用SRBC或HRBC进行同源激发确实能克服免疫抑制。有人提出，每隔4天或6天注射两次抗原从而克服免疫抑制的能力，是由于第一次注射刺激了少量T辅助细胞，第二次注射使这些细胞扩增，从而产生足够的辅助来诱导特异性B细胞反应。
