Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan; International Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Colloids Surf B Biointerfaces. 2024 Nov;243:114125. doi: 10.1016/j.colsurfb.2024.114125. Epub 2024 Jul 25.
Similar to mammalian cells, most bacteria can release nano-sized membrane vesicles (MVs) into the extracellular environment. MVs contain lipids, bioactive proteins, nucleic acids, and metabolites, and play important roles in microbial physiology. MVs have great potential for immunotherapeutic applications, such as bacterial vaccines and cancer immunotherapy. However, because of the diversity in content and heterogeneity in size of MVs, the clinical application of MVs has been limited. Recently, the use of MVs combined with nanoparticles (NPs) has been shown to be effective in improving the homogeneity, stability and function of MVs. In this review, we focus on studies of MVs combined with NPs (MV-NPs) and describe the use of these MV-NPs in biotechnology, especially in bacterial vaccine and cancer immunotherapy.
类似于哺乳动物细胞，大多数细菌可以将纳米大小的膜泡（MVs）释放到细胞外环境中。MVs 含有脂质、生物活性蛋白、核酸和代谢物，并在微生物生理学中发挥重要作用。MVs 在免疫治疗应用方面有很大的潜力，例如细菌疫苗和癌症免疫疗法。然而，由于 MVs 的内容多样性和大小异质性，MVs 的临床应用受到了限制。最近，MVs 与纳米颗粒（NPs）结合的使用已被证明可以有效提高 MVs 的均一性、稳定性和功能。在这篇综述中，我们重点介绍了 MVs 与 NPs 结合的研究（MV-NPs），并描述了这些 MV-NPs 在生物技术中的应用，特别是在细菌疫苗和癌症免疫疗法方面的应用。
