Xu Wei, Maruyama Sayo, Niidome Takuro
Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Institute of Industrial Nanomaterials, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Materials (Basel). 2025 Jul 24;18(15):3470. doi: 10.3390/ma18153470.
serovar Typhimurium (. ) is an intracellular pathogen capable of surviving and replicating within macrophages, which causes foodborne diseases such as gastroenteritis. To develop a strategy against intracellular bacteria in macrophages, we designed silver-loaded biodegradable polymer nanoparticles functionalized with . membrane vesicles (MVs). Silver nanoparticles (Ag NPs) were initially encapsulated within biodegradable poly(lactic-co-glycolic) nanoparticles (Ag-P NPs), which were then surface-modified with polyethyleneimine to form Ag-PP NPs. These were subsequently fused with MVs via a sonication method to generate Ag-PP@MV NPs. The resulting MV-coated nanoparticles displayed a similar protein profile to that of native MVs and exhibited antibacterial activity against intracellular . . Notably, the enhanced cellular uptake of the MV-modified NPs contributed to their intracellular bactericidal efficacy. This study highlights MV modification as a promising strategy to improve NP delivery to macrophages for treating persistent intracellular infections.
鼠伤寒血清型（. ）是一种能够在巨噬细胞内存活和复制的细胞内病原体，可引发如肠胃炎等食源性疾病。为了制定针对巨噬细胞内细菌的策略，我们设计了用. 膜囊泡（MVs）功能化的载银可生物降解聚合物纳米颗粒。银纳米颗粒（Ag NPs）最初被包裹在可生物降解的聚（乳酸 - 乙醇酸）纳米颗粒（Ag - P NPs）中，然后用聚乙烯亚胺进行表面改性以形成Ag - PP NPs。随后通过超声处理方法将这些与MVs融合以生成Ag - PP@MV NPs。所得的MV包被纳米颗粒显示出与天然MVs相似的蛋白质谱，并对细胞内的. 表现出抗菌活性。值得注意的是，MV修饰的纳米颗粒增强的细胞摄取有助于其细胞内杀菌功效。这项研究突出了MV修饰作为一种有前景的策略，可改善纳米颗粒向巨噬细胞的递送，用于治疗持续性细胞内感染。
