Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Key Laboratory of Architectural Cold Climate Energy Management, Ministry of Education, Jilin Jianzhu University, China.
Talanta. 2024 Nov 1;279:126627. doi: 10.1016/j.talanta.2024.126627. Epub 2024 Jul 27.
MiRNA-214 can regulate the expression of their downstream target genes after post-transcriptional and are involved in the biological processes of triple negative breast cancer (TNBC). In this work, the small-sized luminescent NbC nanosheet-based whispering gallery mode-enhanced electrochemiluminescence (ECL) strategy was successfully constructed to detect miRNA-214 in TNBC. Firstly, we have synthesized small-sized luminescent NbC nanosheets from NbAlC MXene. The NbC nanosheets not only exhibited more stable chemical properties and reduced the defects of the large sheet structures, but also possessed the quantum confinement effect with the discrete energy level. As a result, the prepared small-sized NbC nanosheets had unique luminescent and electrochemical properties. Furthermore, in order to improve the ECL performance of NbC nanosheets, SiO microspheres were self-assembled on the electrode surface by gas-liquid interface method to form whispering gallery mode structure. Because the light was continuously reflected at the interface of the microcavity in the whispering gallery mode, the ECL signal of NbC luminescent nanosheets was amplified largely. Finally, the whispering gallery mode-based ECL sensing platform was established. The results showed that the biosensor had a good linear correlation between the ECL intensity and the logarithm of concentration of miRNA-214 in the range of 10 fM to 100 nM with a limit of detection of 2.5 fM. The actual detection of miRNA-214 content in clinical TNBC tissue samples was realized successfully.
miRNA-214 可以在转录后调节其下游靶基因的表达，并参与三阴性乳腺癌（TNBC）的生物过程。在这项工作中，成功构建了基于小型发光 NbC 纳米片的声子回旋模式增强电化学发光（ECL）策略，用于检测 TNBC 中的 miRNA-214。首先，我们从 NbAlC MXene 合成了小型发光 NbC 纳米片。NbC 纳米片不仅表现出更稳定的化学性质和减少了大片结构的缺陷，而且具有量子限制效应和离散能级。因此，制备的小型 NbC 纳米片具有独特的发光和电化学性能。此外，为了提高 NbC 纳米片的 ECL 性能，采用气-液界面法在电极表面自组装 SiO 微球，形成声子回旋模式结构。由于光在微腔的界面处连续反射，NbC 发光纳米片的 ECL 信号被大大放大。最后，建立了基于声子回旋模式的 ECL 传感平台。结果表明，该生物传感器在 10 fM 至 100 nM 的范围内，ECL 强度与 miRNA-214 浓度的对数之间具有良好的线性相关性，检测限为 2.5 fM。成功实现了对临床 TNBC 组织样本中 miRNA-214 含量的实际检测。
