Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University (UAEU), Al Ain, UAE.
Department of Food Science, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
Sci Rep. 2024 Jul 30;14(1):17551. doi: 10.1038/s41598-024-68387-7.
Manuka honey (MH) has garnered much attention due to its remarkable antimicrobial, anticancer, immunomodulatory and wound-healing properties. This study compared the antiproliferative effects of raw and powdered MH (pMH) on various human and murine cancer cell lines. A detailed metabolomics analysis was also carried out using untargeted ultrahigh-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) to compare the constituents in raw MH and pMH. The results of the viability studies showed that both raw MH and pMH caused a dose-dependent inhibition of tumor cell growth at concentrations of > 1% w/v (equivalent to ~ 10 mg/ml). A differential susceptibility to MH was observed among the cell lines with the human MDA-MB-231 and A549 cells and murine B16.F10 cells being relatively resistant to MH while the murine MC38 colorectal adeno-carcinoma cells showing the most sensitivity. The effect of raw MH and pMH on cell viability was validated using 2 indepndent assays. Metabolomics analysis detected 2440 compounds, out of which 833 were successfully identified. Among these, 90 phytochemical compounds, predominantly comprising terpenoids, flavonoids, coumarins and derivatives, and phenylpropanoic acids, and 79 lipids were identifiable. Significant differences in 5 metabolite classes, including flavonoids, phenols, terpenoids, carbohydrates, and organic acids were observed between the raw and pMH. Moreover, several altered metabolic pathways were identified in pMH compared to raw MH, such as energy metabolism, amino acid metabolism, and various other pathways that collectively influence biological functions associated with cellular growth, signaling, and stress response.
麦卢卡蜂蜜（MH）因其具有显著的抗菌、抗癌、免疫调节和伤口愈合特性而备受关注。本研究比较了生麦卢卡蜂蜜（pMH）和粉末状麦卢卡蜂蜜（pMH）对各种人类和鼠类癌细胞系的增殖抑制作用。还使用非靶向超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）进行了详细的代谢组学分析，以比较生麦卢卡蜂蜜和 pMH 中的成分。细胞活力研究的结果表明，生麦卢卡蜂蜜和 pMH 在浓度>1%w/v（相当于~10mg/ml）时，均会引起肿瘤细胞生长的剂量依赖性抑制。细胞系对 MH 的敏感性存在差异，其中人 MDA-MB-231 和 A549 细胞以及鼠 B16.F10 细胞对 MH 相对耐药，而鼠 MC38 结直肠腺癌细胞对 MH 最敏感。使用 2 种独立的检测方法验证了生麦卢卡蜂蜜和 pMH 对细胞活力的影响。代谢组学分析检测到 2440 种化合物，其中 833 种成功鉴定。其中，90 种植物化学物质，主要包括萜类、类黄酮、香豆素及其衍生物和苯丙丙酸，79 种脂质可识别。生麦卢卡蜂蜜和 pMH 之间观察到 5 种代谢物类别的显著差异，包括类黄酮、酚类、萜类、碳水化合物和有机酸。与生麦卢卡蜂蜜相比，pMH 中还鉴定出几种改变的代谢途径，如能量代谢、氨基酸代谢以及与细胞生长、信号转导和应激反应相关的各种其他途径。
