Laboratório de Patologia Clínica Veterinária, Faculdade de Agronomia e Medicina Veterinária (FAV/UnB), Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, Distrito Federal, CEP: 70910-900, Brazil.
Laboratório de Patologia Veterinária, Faculdade de Agronomia e Medicina Veterinária (FAV/UnB), Universidade de Brasília, Brasília, Distrito Federal, Brazil.
Syst Parasitol. 2024 Jul 30;101(5):53. doi: 10.1007/s11230-024-10178-9.
Cytauxzoon spp. have been detected in Brazil infecting mainly asymptomatic domestic cats and wild felids. However, the supposed genetic similarity with the pathogenic Cytauxzoon felis is questionable because it is based on analysis of short sequences of the 18S rRNA gene. Herein, we describe a novel Cytauxzoon species infecting an asymptomatic little-spotted-cat (Leopardus tigrinus) based on morphological, histopathological, and molecular analyses. The animal was attended presenting a history of a run-over with multiple traumas. Although the little-spotted-cat was stabilized, he died a few days later. Ring-shaped merozoites within erythrocytes were found on blood smears and in the abdominal effusion. In addition, schizonts were observed in histiocytes in the liver. Phylogenetic analyses based on both near-complete 18S rRNA and cytb genes positioned the obtained sequences in a unique clade, albeit closely related to Cytauxzoon felis from the USA. Genetic divergences ranging from 0.004 and 0.067-0.068 were found between the near-complete 18S rRNA and cytb sequences of Cytauxzoon sp. detected in the little-spotted-cat and C. felis, respectively. This study evidenced the circulation of a novel Cytauxzoon species, herein named Cytauxzoon brasiliensis sp. nov., in an asymptomatic wild felid species from Brazil. Further studies are necessary to identify Cytauxzoon species from domestic and wild felids in the country.
巴西已检测到 Cytauxzoon spp.，主要感染无症状的家猫和野生猫科动物。然而，与致病性 Cytauxzoon felis 的假定遗传相似性是值得怀疑的，因为它是基于 18S rRNA 基因的短序列分析。在此，我们描述了一种感染无症状小斑点猫（Leopardus tigrinus）的新型 Cytauxzoon 物种，该猫基于形态学、组织病理学和分子分析。该动物因被车撞倒并受多重创伤而接受治疗。尽管小斑点猫得到了稳定，但几天后还是死亡了。在血涂片和腹腔积液中发现了红细胞内的环状裂殖子。此外，还在肝组织细胞中观察到了滋养体。基于近完整的 18S rRNA 和 cytb 基因的系统发育分析将获得的序列定位在一个独特的分支中，尽管与来自美国的 Cytauxzoon felis 密切相关。在小斑点猫中检测到的 Cytauxzoon sp. 的近完整 18S rRNA 和 cytb 序列之间的遗传差异为 0.004 至 0.067-0.068。本研究证明了一种新型 Cytauxzoon 物种（Cytauxzoon brasiliensis sp. nov.）在巴西无症状野生猫科动物中的循环。需要进一步的研究来鉴定巴西国内家猫和野生猫科动物中的 Cytauxzoon 物种。
