Key Laboratory of Functional Polymer Materials of Ministry of Education, College of Chemistry, Nankai University, Tianjin 300071, China.
Department of Oncology, Tianjin Union Medical Center, Nankai University, Tianjin 300191, China.
J Mater Chem B. 2024 Sep 11;12(35):8655-8661. doi: 10.1039/d4tb01062k.
Surgical resection remains the main treatment for malignant tumors. Image-guided surgery aims to remove tumor tissue completely while preserving normal tissue, thereby reducing tumor recurrence rates and injury. However, challenges like tissue autofluorescence, limited probe penetration and low contrast restrict its use. Near-infrared (NIR) persistent luminescent nanoparticles (PLNPs) provide a solution by emitting persistent luminescence (PersL) even after excitation ceases, thus circumventing autofluorescence and enabling deep tumor imaging. In this study, we prepared nano-sized (140 nm hydrodynamic size) Cr doped zinc gallogermanate (ZGC) using a removable template method and modified it with folate acid to obtain ZGC-FA, which exhibits NIR (695 nm) PersL with a signal-to-noise ratio of 23.9 . We utilized a colon cancer model that selectively expressed luciferase for the first time to validate the guiding efficacy of ZGC-FA in precision surgical resection. Post-intraperitoneal injection at 50 minutes, the PersL closely matched the tumor boundaries, achieving an overlap rate of approximately 98%. Complete tumor resection was achieved under PersL guidance, with only 2.3% of healthy tissue removed. This research underscores the potential of ZGC-FA in the field of surgical oncology. The precision of the ZGC-FA guided surgical approach holds promise to enhance surgical outcomes and facilitate postoperative recovery in patients.
手术切除仍然是治疗恶性肿瘤的主要方法。影像引导手术旨在在保留正常组织的同时完全切除肿瘤组织，从而降低肿瘤复发率和损伤。然而，组织自发荧光、探针穿透有限和对比度低等挑战限制了其应用。近红外（NIR）持续发光纳米粒子（PLNPs）通过在激发停止后仍发出持续发光（PersL）来提供解决方案，从而避免了自发荧光，并实现了深层肿瘤成像。在这项研究中，我们使用可去除模板法制备了纳米级（水动力尺寸为 140nm）的 Cr 掺杂锌镓锗酸盐（ZGC），并对其进行了叶酸修饰，得到了 ZGC-FA，其具有 NIR（695nm）PersL，信噪比为 23.9。我们首次利用选择性表达荧光素的结肠癌模型验证了 ZGC-FA 在精确手术切除中的指导效果。在 50 分钟时进行腹腔内注射后，PersL 与肿瘤边界紧密匹配，重叠率约为 98%。在 PersL 引导下实现了完全的肿瘤切除，仅切除了 2.3%的健康组织。这项研究强调了 ZGC-FA 在肿瘤外科领域的潜力。ZGC-FA 引导手术方法的精确性有望提高手术效果，并促进患者术后康复。
