Medical College, Guangxi University, Nanning, Guangxi, China.
Translational Medicine Research Center, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, China.
Environ Toxicol. 2024 Nov;39(11):5074-5085. doi: 10.1002/tox.24390. Epub 2024 Jul 31.
Acrylamide (AAM), a compound extensively utilized in various industrial applications, has been reported to induce toxic effects across multiple tissues in living organisms. Despite its widespread use, the impact of AAM on ovarian function and the mechanisms underlying these effects remain poorly understood. Here, we established an AAM-exposed mouse toxicological model using 21 days of intragastric AAM administration. AAM exposure decreased ovarian coefficient and impaired follicle development. Further investigations revealed AAM would trigger apoptosis and disturb tricarboxylic acid cycle in ovarian tissue, thus affecting mitochondrial electron transport function. Moreover, AAM exposure decreased oocyte and embryo development potential, mechanically associated with pericentrin and phosphorylated Aurora A cluster failure, leading to meiotic spindle assembly defects. Collectively, these results suggest that AAM exposure may lead to apoptosis, glucose metabolic disorders, and mitochondrial dysfunction in ovary tissue, ultimately compromising oocyte quality.
丙烯酰胺（AAM）是一种广泛应用于各种工业应用的化合物，据报道，它会在生物体的多种组织中引起毒性作用。尽管它的用途广泛，但 AAM 对卵巢功能的影响及其潜在机制仍知之甚少。在这里，我们使用 21 天的腹腔内 AAM 给药建立了一个 AAM 暴露的小鼠毒理学模型。AAM 暴露降低了卵巢系数并损害了卵泡发育。进一步的研究表明，AAM 会引发卵巢组织中的细胞凋亡并扰乱三羧酸循环，从而影响线粒体电子传递功能。此外，AAM 暴露降低了卵母细胞和胚胎的发育潜力，这与中心体蛋白和磷酸化 Aurora A 簇的故障有关，导致减数分裂纺锤体组装缺陷。总的来说，这些结果表明，AAM 暴露可能导致卵巢组织中的细胞凋亡、葡萄糖代谢紊乱和线粒体功能障碍，最终损害卵母细胞质量。
