Kim Min Jae, Cheng Zhihong
Department of Natural Medicine, School of Pharmacy, Fudan University, Shanghai, China.
Nat Prod Res. 2024 Jul 31:1-6. doi: 10.1080/14786419.2024.2385697.
sp., an endophytic fungi found in the medicinal plant of leaves, was reported to possess antimicrobial compounds from its unidentified species. In this study, 21 cyclic dipeptides (-) were isolated and identified from . All the 21 isolated cyclic dipeptides were reported for the first time from the genus . The antimicrobial activity by the disc diffusion method showed that five compounds including cyclo(Pro-Gly) (), cyclo(Phe-Ile) (), cyclo(Ile-Val) (), cyclo(Val-Ala) (), and cyclo(Ala-Phe) () inhibited the growth of with inhibition zone diameters ranging from 12 to 30 mm. Further bioassay demonstrated that four cyclic dipeptides (, , , and ) showed significant antibacterial activity against with MIC values of 0.04, 0.39, 0.01, and 0.10 µg/mL, respectively, as compared to the positive control (ciprofloxacin, MIC = 0.08 µg/mL). However, none of these cyclic dipeptides showed obvious anti-fungal activity against .
某内生真菌，发现于一种药用植物的叶子中，据报道其未鉴定的物种含有抗菌化合物。在本研究中，从该真菌中分离并鉴定出21种环二肽（-）。所有21种分离出的环二肽均首次从该属中报道。采用纸片扩散法进行抗菌活性检测，结果表明，包括环（脯氨酸-甘氨酸）（）、环（苯丙氨酸-异亮氨酸）（）、环（异亮氨酸-缬氨酸）（）、环（缬氨酸-丙氨酸）（）和环（丙氨酸-苯丙氨酸）（）在内的5种化合物抑制了某菌的生长，抑菌圈直径为12至30毫米。进一步的生物测定表明，与阳性对照（环丙沙星，MIC = 0.08μg/mL）相比，4种环二肽（、、、和）对某菌显示出显著的抗菌活性，MIC值分别为0.04、0.39、0.01和0.10μg/mL。然而，这些环二肽均未对某真菌显示出明显的抗真菌活性。
