鉴定芸薹根肿菌致病型的五个分子标记的特征。
Characterization of Five Molecular Markers for Pathotype Identification of the Clubroot Pathogen Plasmodiophora brassicae.
机构信息
First, second, third, fifth, sixth, seventh, and ninth authors: College of Agronomy; fourth author: College of Resources; and eighth author: Rice Research Institute and Research Center for Major Crop Diseases, Sichuan Agricultural University Chengdu Campus, Chengdu 611130, China.
出版信息
Phytopathology. 2018 Dec;108(12):1486-1492. doi: 10.1094/PHYTO-11-17-0362-R. Epub 2018 Nov 5.
Clubroot disease is an important disease on cruciferous crops caused by Plasmodiophora brassicae infections. The pathotypes have been classified based on the reactions of differential hosts. However, molecular markers of particular pathotypes for P. brassicae are limited. In this study, we found five genetic markers in association with different pathotypes. Different gene expression patterns among different pathotypes (P4, P7, P9, and P11) were assayed according to the transcriptome data. The assay indicated that molecular markers PBRA_007750 and PBRA_009348 could be used to distinguish P11 from P4, P7, and P9; PBRA_009348 and Novel342 could distinguish P9 from P4, P7, and P11; and PBRA_008439 and Novel342 could represent a kind of P4. Polymerase chain reaction cycles ranging from 25 to 30 were able to identify the predominant pathotype in general. Therefore, these molecular markers would be a valuable tool to identify and discriminate pathotypes in P. brassicae population.
根肿病是十字花科作物的重要病害，由芸薹根肿菌引起。根据鉴别寄主的反应对致病型进行了分类。然而，芸薹根肿菌特定致病型的分子标记有限。本研究发现了与不同致病型相关的五个遗传标记。根据转录组数据检测了不同致病型（P4、P7、P9 和 P11）之间的不同基因表达模式。结果表明，分子标记 PBRA_007750 和 PBRA_009348 可用于区分 P11 与 P4、P7 和 P9；PBRA_009348 和 Novel342 可区分 P9 与 P4、P7 和 P11；而 PBRA_008439 和 Novel342 可代表一种 P4。聚合酶链反应循环数在 25 到 30 之间可以普遍鉴定出主要的致病型。因此，这些分子标记将成为鉴定和区分芸薹根肿菌种群中致病型的有价值工具。
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