Faculty of Pharmacy, Department of Biochemistry, Harran University, Şanlıurfa, Turkey.
Department of Pharmacy Services, Vocational School of Health Services, Tarsus University, Mersin, Turkey.
Biochem Biophys Res Commun. 2024 Nov 19;734:150464. doi: 10.1016/j.bbrc.2024.150464. Epub 2024 Jul 27.
Breast cancer is most common cancer among women in the World. Thymoquinone (TQ) exhibits a wide range of biological activities such as anticancer, antidiabetic, antimicrobial, analgesic, antioxidant, and anti-inflammatory effects. However, its effectiveness in cancer treatment is hindered by its poor bioavailability, attributed to its limited solubility in water. Hence, novel strategies are required to enhance the bioavailability of TQ, which possesses remarkable anticancer characteristics. The aim of this study is to prepare pHEMA-based magnetic nanoparticles carrying TQ (TQ-MNPs) to improve bioavailability, and therapeutic efficacy against breast cancer. For this purpose, TQ-MNPs were synthesized and characterized with Fourier transform infrared spectrophotometer (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS), magnetic field using a vibrating sample magnetometer (VSM). The loading capabilities of synthesized magentic nanostructures were evaluated, and release investigations were conducted under experimental conditions that mimic the cellular environment. The findings of the studies indicated that the TQ carrying capacity of MNPs was deemed satisfactory, and the release efficiency was adequate. MNPs and TQ-MNPs showed biocompatibility against HDFa cells. TQ-MNPs showed stronger anti-proliferative activity against MCF-7 breast cancer cells compared to free TQ (p < 0.05). TQ-MNPs induced apoptosis in MCF-7 breast cancer cells.
乳腺癌是全世界女性中最常见的癌症。姜黄素（TQ）表现出广泛的生物活性，如抗癌、抗糖尿病、抗菌、镇痛、抗氧化和抗炎作用。然而，其在癌症治疗中的有效性受到其生物利用度差的限制，这归因于其在水中的溶解度有限。因此，需要新的策略来提高 TQ 的生物利用度，TQ 具有显著的抗癌特性。本研究旨在制备载有 TQ 的 pHEMA 基磁性纳米粒子（TQ-MNPs），以提高生物利用度，并提高治疗乳腺癌的疗效。为此，用傅里叶变换红外分光光度计（FTIR）、扫描电子显微镜（SEM）、动态光散射（DLS）、振动样品磁强计（VSM）对 TQ-MNPs 进行了合成和表征。评估了合成磁纳米结构的载药能力，并在模拟细胞环境的实验条件下进行了释放研究。研究结果表明，MNPs 的载药量令人满意，释放效率也足够。MNPs 和 TQ-MNPs 对 HDFa 细胞表现出良好的生物相容性。与游离 TQ 相比，TQ-MNPs 对 MCF-7 乳腺癌细胞表现出更强的抗增殖活性（p < 0.05）。TQ-MNPs 诱导 MCF-7 乳腺癌细胞凋亡。
