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用于检测辛德毕斯病毒免疫球蛋白M抗体的灵敏酶免疫测定法以及波戈斯塔病由西部马脑炎复合病毒引起的进一步证据。

Sensitive enzyme immunoassay for detecting immunoglobulin M antibodies to Sindbis virus and further evidence that Pogosta disease is caused by a western equine encephalitis complex virus.

作者信息

Calisher C H, Meurman O, Brummer-Korvenkontio M, Halonen P E, Muth D J

出版信息

J Clin Microbiol. 1985 Oct;22(4):566-71. doi: 10.1128/jcm.22.4.566-571.1985.

Abstract

An antibody capture enzyme immunoassay (EIA) was adapted for the detection of immunoglobulin M (IgM) antibody to Sindbis (SIN) virus. Sera from humans with a febrile illness characterized by rash and arthralgia in eastern Finland (Pogosta [POG] disease) and Sweden (Ockelbo disease) and from humans with western equine encephalitis (WEE) virus infection in the United States were tested for IgM antibodies by EIA. Seroconversions were documented in patients with POG disease and with WEE virus infections by using SIN virus as antigen and rabbit anti-SIN virus immunoglobulin; this confirms previous observations that POG disease is caused by a virus closely related to SIN virus and that IgM antibodies to WEE complex alphaviruses are not type specific. This IgM EIA provided a sensitive diagnostic and research tool applicable to epidemiologic problems posed by POG disease.

摘要

一种抗体捕获酶免疫测定法(EIA)被用于检测抗辛德毕斯(SIN)病毒的免疫球蛋白M(IgM)抗体。通过EIA检测了来自芬兰东部(波戈斯塔[POG]病)和瑞典(奥克尔博病)患有以皮疹和关节痛为特征的发热性疾病的人类血清,以及来自美国患有西部马脑炎(WEE)病毒感染的人类血清中的IgM抗体。通过使用SIN病毒作为抗原和兔抗SIN病毒免疫球蛋白,记录了POG病患者和WEE病毒感染患者的血清转化情况;这证实了先前的观察结果,即POG病由一种与SIN病毒密切相关的病毒引起,并且针对WEE复合α病毒的IgM抗体不是型特异性的。这种IgM EIA提供了一种灵敏的诊断和研究工具,适用于由POG病引发的流行病学问题。

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