在蚊虫样本中检测拉克罗斯虫媒病毒抗原：显色和荧光酶免疫分析系统的应用。
Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.
作者信息
Hildreth S W, Beaty B J, Meegan J M, Frazier C L, Shope R E
出版信息
J Clin Microbiol. 1982 May;15(5):879-84. doi: 10.1128/jcm.15.5.879-884.1982.
Abstract
An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h after the addition of test samples. La Crosse viral antigen could be readily detected in mosquito pools after seven freeze-thaw cycles.
摘要
开发并评估了一种产生显色或荧光终产物的酶免疫测定法，用于检测蚊群中的拉克罗斯病毒抗原。发现该酶免疫测定法灵敏，能在100只蚊子的蚊群中检测出一只受感染的蚊子，且具有特异性，能区分密切相关的加利福尼亚群病毒。在加入测试样品后5小时内即可完成测定。经过7次冻融循环后，仍能在蚊群中轻松检测到拉克罗斯病毒抗原。
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本文引用的文献
1
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Yale J Biol Med. 1980 Jan-Feb;53(1):85-92.
2
Detection of attograms of antigen by a high-sensitivity enzyme-linked immunoabsorbent assay (HS-ELISA) using a fluorogenic substrate.使用荧光底物的高灵敏度酶联免疫吸附测定（HS-ELISA）检测抗原的阿托克量级。
J Immunol Methods. 1980;38(1-2):125-39. doi: 10.1016/0022-1759(80)90337-3.
3
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Yale J Biol Med. 1980 Jan-Feb;53(1):93-9.
4
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J Clin Microbiol. 1980 Nov;12(5):704-8. doi: 10.1128/jcm.12.5.704-708.1980.
5
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6
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7
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8
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9
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10
[Enzyme immunoassay for the detection and identification of dengue viruses from infected mosquitoes (author's transl)].
Ann Microbiol (Paris). 1979 Oct;130B(3):363-74.
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