Biochemistry & Toxicology Division, Department of Zoology, University of Calicut, Malappuram, Kerala 673 635, India.
Pestic Biochem Physiol. 2024 Aug;203:106023. doi: 10.1016/j.pestbp.2024.106023. Epub 2024 Jul 9.
Acephate and chlorantraniliprole are two insecticides widely used in agricultural applications. Several studies were focused on the mode of action and related biological and cellular level expressions. However, the sub-lethal dose and related molecular expression level of acephate and chlorantraniliprole have not been evaluated or studied to the same degree. In this study, we investigated the sub-lethal toxicity of acephate and chlorantraniliprole in Drosophila melanogaster. The EC value was recorded with high difference, and is found to be 1.9 μg/ml and 0.029 μg/ml respectively for acephate and chlorantraniliprole, the difference is simply because of the different modes of action. The 1/5th EC concentration was selected for studying the pesticide induced transcriptomics in D. melanogaster. Both pesticides significantly altered the expression profile of several transcripts which are involved in proteolysis, detoxification, chromosome associated proteins and immune response genes and so on. The effect of both pesticides on D. melanogaster was further explored by screening the genes involved in toxicity, which were analyzed using, GO and KEGG pathways. The results revealed that the sub-lethal exposure of both pesticides caused significant changes in the global gene transcription profiles and each pesticide had their unique mode of alteration in the D. melanogaster.
乙酰甲胺磷和氯虫苯甲酰胺是两种广泛应用于农业领域的杀虫剂。已有多项研究集中于这两种杀虫剂的作用模式以及相关的生物和细胞水平表达。然而，乙酰甲胺磷和氯虫苯甲酰胺的亚致死剂量及其相关的分子表达水平尚未得到同等程度的评估或研究。在本研究中，我们研究了乙酰甲胺磷和氯虫苯甲酰胺对黑腹果蝇的亚致死毒性。实验记录到了两者 EC 值的显著差异，乙酰甲胺磷和氯虫苯甲酰胺的 EC 值分别为 1.9μg/ml 和 0.029μg/ml，这仅仅是因为两者的作用模式不同。我们选择了 1/5 EC 浓度来研究农药诱导的黑腹果蝇转录组学变化。这两种杀虫剂都显著改变了几个参与蛋白水解、解毒、染色体相关蛋白和免疫反应基因等转录本的表达谱。通过筛选涉及毒性的基因，进一步研究了这两种杀虫剂对黑腹果蝇的影响，利用 GO 和 KEGG 途径对这些基因进行了分析。结果表明，两种杀虫剂的亚致死暴露导致了黑腹果蝇整体基因转录谱的显著变化，并且每种杀虫剂在黑腹果蝇中都有其独特的改变模式。
