Graduate Program in Veterinary Sciences, Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
Enzymology Laboratory, Department of Animal Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
J Comp Physiol B. 2024 Dec;194(6):793-804. doi: 10.1007/s00360-024-01577-x. Epub 2024 Jul 31.
The present study aimed to establish zebrafish as an experimental model for investigations into obesity and physical exercise, as well as to assess the effects of these factors on metabolism. The experiment spanned twelve weeks, comprising a feeding trial during which the last four weeks incorporated a physical exercise protocol. This protocol involved placing fifteen animals in a five-liter aquarium, where they were subjected to swimming at an approximate speed of 0.08 m/s for 30 min daily. Throughout the experiment, histological analyses of visceral, subcutaneous, and hepatic adipose tissues were conducted, along with biochemical analyses of total cholesterol and its fractions, triglycerides, glucose, lactate, and alanine aminotransferase (ALT) levels. Additionally, oxidative stress markers, such as reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and catalase activity and the formation of thiobarbituric acid-reactive substances, were investigated. The results revealed that the group fed a high-fat diet exhibited an increase in ROS production and SOD activity. In contrast, the group administered the high-fat diet and subjected to physical exercise demonstrated a notable reduction in visceral adipocyte area, hepatic steatosis levels, ALT levels, and SOD activity. These findings indicate that physical exercise has a positive effect on obesity and oxidative stress in zebrafish, providing promising evidence for future investigations in this field.
本研究旨在建立斑马鱼作为肥胖和体育锻炼研究的实验模型，并评估这些因素对新陈代谢的影响。实验持续了十二周，包括喂养试验，其中最后四周包含体育锻炼方案。该方案涉及将十五只动物置于五升的水族箱中，让它们以 0.08 m/s 的近似速度每天游泳 30 分钟。在整个实验过程中，对内脏、皮下和肝脂肪组织进行了组织学分析，并对总胆固醇及其各组分、甘油三酯、葡萄糖、乳酸和丙氨酸氨基转移酶（ALT）水平进行了生化分析。此外，还研究了氧化应激标志物，如活性氧（ROS）水平、超氧化物歧化酶（SOD）活性和过氧化氢酶活性以及硫代巴比妥酸反应物质的形成。结果表明，高脂饮食组的 ROS 产生和 SOD 活性增加。相比之下，给予高脂饮食并进行体育锻炼的组，内脏脂肪细胞面积、肝脂肪变性水平、ALT 水平和 SOD 活性显著降低。这些发现表明，体育锻炼对斑马鱼的肥胖和氧化应激有积极影响，为该领域的未来研究提供了有希望的证据。
