Dandin Esra, Üstündağ Ünsal Veli, Ünal İsmail, Ateş-Kalkan Perihan Seda, Cansız Derya, Beler Merih, Ak Esin, Alturfan A Ata, Emekli-Alturfan Ebru
Department of Biochemistry, Institute of Health Sciences, Marmara University, Istanbul, Turkey.
Department of Biochemistry, Faculty of Medicine, Istanbul Medipol University, Kavacık, Istanbul, Turkey.
Obes Res Clin Pract. 2022 Jan-Feb;16(1):23-29. doi: 10.1016/j.orcp.2022.01.002. Epub 2022 Jan 12.
Obesity is an independent risk factor for type 2 diabetes and epigenetic regulatory mechanisms affect obesity-related mechanisms. Due to weight gain concern in society, artificial sweeteners with no nutritional value have been increasingly consumed. Stevia is a sweet natural glycoside and a calorie-free sweetner extracted from the leaves of Stevia rebaudiana Bertoni and used as a substitute for artificial sweetners. This study evaluates the effects of stevioside on glucose tolerance, epigenetic and metabolic regulators of insulin resistance, oxidant-antioxidant status and tissue histology in a diet-induced obese (DIO) zebrafish model. After 15 days of overfeeding body weight, and fasting blood glucose, lipid peroxidation and nitric oxide levels and the expressions of fbf21, lepa, ll21, tnfα were elevated, where as there was impaired glucose tolerance and lower superoxide dismutase and glutathione S-transferase activities, dnmt3a expression which is an epigenetic tool of insulin resistance. Beneficial effects of stevioside were observed on glucose tolerance, oxidative stress and inflammatory mediators linking obesity to insulin resistance and its epigenetic regulation, in DIO model.
肥胖是2型糖尿病的独立危险因素，表观遗传调控机制影响与肥胖相关的机制。由于社会对体重增加的关注，无营养价值的人工甜味剂的消费量日益增加。甜菊糖苷是一种天然甜味糖苷，是从甜叶菊的叶子中提取的无热量甜味剂，用作人工甜味剂的替代品。本研究评估了甜菊糖苷对饮食诱导肥胖（DIO）斑马鱼模型的葡萄糖耐量、胰岛素抵抗的表观遗传和代谢调节因子、氧化-抗氧化状态及组织组织学的影响。过度喂养15天后，体重、空腹血糖、脂质过氧化和一氧化氮水平以及fbf21、lepa、ll21、tnfα的表达均升高，而葡萄糖耐量受损，超氧化物歧化酶和谷胱甘肽S-转移酶活性降低，dnmt3a表达降低，dnmt3a是胰岛素抵抗的一种表观遗传工具。在DIO模型中，观察到甜菊糖苷对葡萄糖耐量、氧化应激以及将肥胖与胰岛素抵抗及其表观遗传调控联系起来的炎症介质具有有益作用。
