NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam.
Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.
J Microbiol Biotechnol. 2024 Aug 28;34(8):1680-1687. doi: 10.4014/jmb.2402.02052. Epub 2024 Jul 12.
The diverse pharmacological properties of edible bird's nest (EBN) have been elucidated in recent years; however, investigations into its antibacterial effects are still limited. In the present study, we explored the antibacterial activity of a peptide-rich extract of EBN against , a notorious pathogen. The EBN extract (EEE) was prepared by soaking EBN in 80% ethanol for 2 days at 60°C. Biochemical analyses showed that peptides at the molecular weight range of 1.7-10 kDa were the major biochemical compounds in the EEE. The extract exhibited strong inhibition against at a minimum inhibitory concentration (MIC) of 125 μg/ml and a minimum bactericidal concentration (MBC) of 250 μg/ml. This activity could be attributed to the impact of the extract on cell membrane integrity and potential, biofilm formation, and reactive oxidative species (ROS) production. Notably, the expression of biofilm- and ROS-associated genes, including (), , , , and (), were deregulated in upon the extract treatment. Our findings indicate a noteworthy pharmacological activity of EBN that could have potential application in the control of .
近年来，已阐明食用燕窝（EBN）的多种药理学特性；然而，其抗菌作用的研究仍然有限。在本研究中，我们探索了富含肽的 EBN 提取物对 ，一种臭名昭著的病原体的抗菌活性。EBN 提取物（EEE）通过将 EBN 在 60°C 下用 80%乙醇浸泡 2 天来制备。生化分析表明，EEE 中的主要生化化合物是分子量在 1.7-10 kDa 范围内的肽。该提取物在最低抑菌浓度（MIC）为 125 μg/ml 和最低杀菌浓度（MBC）为 250 μg/ml 时对 表现出强烈的抑制作用。这种活性可能归因于提取物对细胞膜完整性和潜在的影响，生物膜形成和活性氧物质（ROS）的产生。值得注意的是，在提取物处理后，与生物膜和 ROS 相关的基因，包括 （）、 （）、 （）、 （）和 （）的表达在 中被下调。我们的研究结果表明 EBN 具有显著的药理学活性，可能在控制 方面具有潜在的应用。
