Bempong M A, Mantley R
J Environ Pathol Toxicol Oncol. 1985 Nov-Dec;6(2):293-301.
The toxicity and mutagenicity of 1,3-diphenylguanidine (DPG) were monitored in Salmonella bacteria using the direct incorporation protocol. The test consisted of either direct incorporation of DPG or analysis of body fluid and faecal material derived from animals exposed to DPG. The data from direct incorporation of DPG suggested that the compound is a direct acting mutagen and that in the presence of metabolic activation system there was a reduction in the number of histidine revertants. The data further showed that while higher dosage levels of DPG without metabolic activation were extremely toxic and thus generated significantly fewer revertants, similar concentrations proved to be moderately mutagenic in the presence of S-9 mix. From the data on mutagenic activity of body fluids and faeces, it is suggested that (1) the rate of excretion of i.p.-administered DPG into extracellular fluids, urinary and gastrointestinal tracts is concentration dependent, and (2) a greater proportion of the compound is eliminated through the urinary tract.
采用直接掺入法在沙门氏菌中监测1,3 - 二苯基胍（DPG）的毒性和致突变性。该试验包括直接掺入DPG或分析暴露于DPG的动物的体液和粪便物质。DPG直接掺入的数据表明该化合物是一种直接作用的诱变剂，并且在存在代谢激活系统的情况下，组氨酸回复突变体的数量减少。数据进一步表明，虽然在没有代谢激活的情况下较高剂量水平的DPG具有极高的毒性，因此产生的回复突变体明显较少，但在存在S - 9混合物的情况下，类似浓度的DPG被证明具有中等致突变性。从体液和粪便的诱变活性数据来看，表明：（1）腹腔注射的DPG排泄到细胞外液、尿液和胃肠道的速率取决于浓度，（2）该化合物的更大比例通过泌尿道排出。
