Integrating Pt nanoparticles with 3D Cu Se/GO nanostructure to achieve nir-enhanced peroxidizing Nano-enzymes for dynamic monitoring the level of HO during the inflammation.

The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, HO serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu Se/GO nanostructure-based nanonzymatic HO electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu Se/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu Se nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu Se NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu Se/GO@Pt/SPCE sensing electrode for HO (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables real-time monitoring of HO released by cells. The NIR-enhanced Cu Se/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (HO、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.