Mirderikvand Atefeh, Shahsavari Gholamreza, Moayyed Kazemi Alireza, Ahmadpour Fatemeh, Yalameha Banafsheh
Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Internal Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Rep Biochem Mol Biol. 2024 Jan;12(4):631-642. doi: 10.61186/rbmb.12.4.631.
Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-125b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription 3 (STAT3) and sirtuin 6 (SIRT6) in AS. This study aimed to survey the expression levels of miR-125b, STAT3, and SIRT6 in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors.
This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR.
The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05).
STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes.
动脉粥样硬化（AS）是一种与血管事件相关的炎症性疾病，微小RNA（miR）-125b失调会促进心血管疾病的发病机制。此外，有证据表明信号转导和转录激活因子3（STAT3）和沉默调节蛋白6（SIRT6）参与了AS的发生。本研究旨在调查AS患者和对照组外周血单个核细胞（PBMC）中miR-125b、STAT3和SIRT6的表达水平，并找出它们与生化参数和危险因素的相关性。
本研究纳入了45名对照组和45名AS患者的血样，使用Ficoll溶液分离PBMC。通过定量实时聚合酶链反应测定miR-125b、STAT3和SIRT6的表达水平。
研究结果显示，与对照组相比，患者的miR-125b水平显著升高（P = 0.017）。然而，STAT3和SIRT6表达的变化并不显著（P>0.05）。miR-125b与STAT3（P = 0.522）或SIRT6（P = 0.88）之间没有显著关系。miR-125b与致动脉粥样硬化指数和肌酐呈显著关系（P<0.05），而SIRT6与高密度脂蛋白和肌酐的关联显著（P<0.05）。STAT3在识别心脏病和高血压风险个体方面具有较高的诊断能力（P<0.05）。
STAT3可作为检测AS及AS相关危险因素的有价值生物标志物。miR-125b和SIRT6可能与AS脂质代谢有关。然而，建议进行更大样本量的进一步研究，以从机制上阐明这些基因之间的关联。
