Basaksehir Cam and Sakura City Hospital, Department of Algology, Istanbul, Türkiye.
Turk Neurosurg. 2024;34(5):856-864. doi: 10.5137/1019-5149.JTN.43768-23.3.
To evaluate the effects of tramadol on inflammation by measuring NLRP1 and IL-1 beta (IL-1β) levels in an experimental neuropathic pain model.
Sprague-Dawley rats were divided into three groups: control, chronic constriction injury (CCI), and CCI + tramadol. Neuropathic pain was assessed using mechanical allodynia, thermal hyperalgesia, and cold allodynia. IL-1β and NLRP1 levels were evaluated using ELISA on sciatic nerve (SN), dorsal root ganglion (DRG), and serum either on day 3 or days 8 postsurgery.
On day 3, paw withdrawal latency (PWL) was lower in the CCI and CCI + tramadol groups than the control group in both mechanical and cold allodynia tests. On day 8, the PWL in the CCI group was also lower than in the control group. In contrast, tramadol increased the PWL on day 8 compared to day 3 in the CCI group. During cold allodynia, PWL decreased in the CCI group, however, tramadol reversed this effect on days 3 and 8. Tramadol, therefore, ameliorated pain hypersensitivity in mechanical/cold allodynia tests. Serum IL-1β levels were higher in the CCI + tramadol and CCI groups than the control group, although serum IL-1β levels in the CCI and CCI + tramadol groups were comparable. Tramadol decreased the IL-1β and NLRP1 in DRG compared with the CCI group. A similar trend was observed in the SN samples.
Our experiments revealed an increase in IL-1β and NLRP-1 levels in a neuropathic pain model and found that tramadol had an anti-inflammatory effect on the IL-1β and NLRP1 inflammasomes.
通过测量实验性神经病理性疼痛模型中 NLRP1 和白细胞介素-1β（IL-1β）的水平来评估曲马多对炎症的影响。
将 Sprague-Dawley 大鼠分为三组：对照组、慢性缩窄性损伤（CCI）组和 CCI+曲马多组。通过机械性痛觉过敏、热痛觉过敏和冷痛觉过敏评估神经病理性疼痛。术后第 3 天或第 8 天，通过 ELISA 评估坐骨神经（SN）、背根神经节（DRG）和血清中的 IL-1β和 NLRP1 水平。
第 3 天，机械性和冷痛觉过敏测试中，CCI 组和 CCI+曲马多组的足底退缩潜伏期（PWL）均低于对照组。第 8 天，CCI 组的 PWL 也低于对照组。相比之下，CCI 组在第 8 天的 PWL 高于第 3 天。在冷痛觉过敏期间，CCI 组的 PWL 下降，但曲马多在第 3 天和第 8 天逆转了这种作用。因此，曲马多改善了机械性/冷痛觉过敏测试中的疼痛过敏。CCI+曲马多组和 CCI 组的血清 IL-1β 水平均高于对照组，尽管 CCI 组和 CCI+曲马多组的血清 IL-1β 水平相当。与 CCI 组相比，曲马多降低了 DRG 中的 IL-1β 和 NLRP1。SN 样本中也观察到类似的趋势。
我们的实验表明，神经病理性疼痛模型中 IL-1β 和 NLRP1 水平升高，并发现曲马多对 IL-1β 和 NLRP1 炎性小体具有抗炎作用。
