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咬痕微生物组分析与法医DNA分型的整合:进展、挑战及协同方法

Integration of Bite Mark Microbiome Analysis with Forensic DNA Profiling: Advancements, Challenges, and Synergistic Approaches.

作者信息

Mehar Palash Arun, Bhoyar Lina Zamsingh, Mahakalkar Archana Laxminarayan

机构信息

Department of Forensic Medicine & Toxicology, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India.

Department of Forensic Biology, Government Institute of Forensic Science, Civil Lines, Nagpur, Maharashtra, India.

出版信息

Rambam Maimonides Med J. 2024 Jul 30;15(3):e0014. doi: 10.5041/RMMJ.10528.

DOI:10.5041/RMMJ.10528
PMID:39088706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11294684/
Abstract

Bite mark analysis plays a pivotal role in forensic investigations, by helping to identify suspects and establish links between individuals and crime scenes. However, traditional bite mark methodologies face significant challenges due to issues with reliability and subjectivity. Recent advances in microbiome analysis, which involves identifying and characterizing the microbial communities found in bite marks, have led to the emergence of a promising tool for forensic investigations. The integration of microbiome analysis with conventional DNA profiling enables more accurate interpretation of bite mark evidence in forensic investigations. This review provides an in-depth look at the integration of bite mark microbiome analysis with forensic DNA profiling. It also addresses the challenges and strategies involved in microbiome-based bite mark analysis for forensic purposes.

摘要

咬痕分析在法医调查中起着关键作用,有助于识别嫌疑人并建立个人与犯罪现场之间的联系。然而,由于可靠性和主观性问题,传统的咬痕分析方法面临重大挑战。微生物组分析的最新进展,即识别和表征咬痕中发现的微生物群落,催生了一种用于法医调查的有前景的工具。将微生物组分析与传统的DNA分析相结合,能够在法医调查中更准确地解读咬痕证据。本综述深入探讨了咬痕微生物组分析与法医DNA分析的整合。它还讨论了基于微生物组的咬痕法医分析所涉及的挑战和策略。

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Rambam Maimonides Med J. 2024 Jul 30;15(3):e0014. doi: 10.5041/RMMJ.10528.
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本文引用的文献

1
Reverse engineering in forensic investigations: a new approach to bite mark analysis.法医调查中的逆向工程:一种新的咬痕分析方法。
J Forensic Odontostomatol. 2023 Dec 27;41(3):45-51.
2
Salivary microbiomes: a potent evidence in forensic investigations.唾液微生物组:法医学调查中的有力证据。
Forensic Sci Med Pathol. 2024 Sep;20(3):1058-1065. doi: 10.1007/s12024-023-00759-3. Epub 2024 Jan 4.
3
The Future Is Now: Unraveling the Expanding Potential of Human (Necro)Microbiome in Forensic Investigations.未来已来:揭示人类(坏死)微生物群在法医调查中不断扩大的潜力。
Microorganisms. 2023 Oct 7;11(10):2509. doi: 10.3390/microorganisms11102509.
4
Forensic imaging: a powerful tool in modern forensic investigation.法医成像:现代法医调查中的强大工具。
Forensic Sci Res. 2022 Mar 7;7(3):385-392. doi: 10.1080/20961790.2021.2008705. eCollection 2022.
5
Forensic microbiology and bite marks: a systematic review.法医微生物学与咬痕:系统评价。
J Forensic Odontostomatol. 2022 Aug 30;40(2):44-51.
6
At the Interface of Life and Death: Post-mortem and Other Applications of Vaginal, Skin, and Salivary Microbiome Analysis in Forensics.在生死交界处:阴道、皮肤和唾液微生物组分析在法医学中的尸检及其他应用
Front Microbiol. 2021 Jul 28;12:694447. doi: 10.3389/fmicb.2021.694447. eCollection 2021.
7
A systematic review of 3D scanners and computer assisted analyzes of bite marks: searching for improved analysis methods during the Covid-19 pandemic.三维扫描仪和计算机辅助咬痕分析的系统评价:在新冠疫情期间寻找改进的分析方法。
Int J Legal Med. 2022 Jan;136(1):209-217. doi: 10.1007/s00414-021-02667-z. Epub 2021 Jul 24.
8
Current challenges and best-practice protocols for microbiome analysis.当前微生物组分析面临的挑战和最佳实践方案。
Brief Bioinform. 2021 Jan 18;22(1):178-193. doi: 10.1093/bib/bbz155.
9
DNA extraction for human microbiome studies: the issue of standardization.人类微生物组研究中的 DNA 提取:标准化问题。
Genome Biol. 2019 Oct 21;20(1):212. doi: 10.1186/s13059-019-1843-8.
10
Salivary signature in forensic profiling: A scoping review.法医分析中的唾液特征:一项范围综述。
J Forensic Dent Sci. 2018 Sep-Dec;10(3):123-127. doi: 10.4103/jfo.jfds_30_18.