Smirnov S V, Malygin A G
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1661-8.
The qualitative and quantitative characteristics of the short oligonucleotides synthesis by Escherichia coli RNA polymerase on A1 promoter of the bacteriophage T7 in the presence if incomplete set of nucleoside triphosphates were studied. The binding of the fourth substrate with enzyme-template complex was shown to occur after binding of the third substrate only. The curves of di-, tri- and tetranucleotide synthesis as the function of CTP and GTP concentration were constructed. The empiric formulas for the rates of the coupled synthesis if tri- and tetranucleotides were derived from these curves. A kinetic scheme describing the experimental data was proposed.
研究了在核苷三磷酸不完全的情况下，大肠杆菌RNA聚合酶在噬菌体T7的A1启动子上合成短寡核苷酸的定性和定量特征。结果表明，只有在第三个底物结合后，第四个底物才会与酶-模板复合物结合。构建了二核苷酸、三核苷酸和四核苷酸合成速率随CTP和GTP浓度变化的曲线。从这些曲线中得出了三核苷酸和四核苷酸偶联合成速率的经验公式。提出了一个描述实验数据的动力学方案。
