Grachev M A, Zaĭchikov E F, Pletnev A G, Starikov V V
Mol Biol (Mosk). 1980 May-Jun;14(3):575-85.
Characteristics of NTP gamma-anilidates (AnpppN) as substrates of E. coli RNA-polymerase have been studied. Michaelis constants for AnpppN are about an order of magnitude greater than those for the usual substrates, whereas the maximum rates differ but unsignificantly. AnpppA is almost completely transformed to polyA and Anppi when synthesis of polyA takes place with AnpppA as the only substrate with denatured DNA as template (reiteration system). The AnpppA-residue is present at the 5'-terminus of RNA synthesized from AnpppA, CTP, UTP and GTP on T7 DNA template by the holo-enzyme.
已对作为大肠杆菌RNA聚合酶底物的NTPγ-酰苯胺（AnpppN）的特性进行了研究。AnpppN的米氏常数比通常底物的米氏常数大约高一个数量级，而最大反应速率虽有差异但不显著。当以AnpppA作为唯一底物、变性DNA为模板进行聚腺苷酸（polyA）合成（重复系统）时，AnpppA几乎完全转化为聚A和Anppi。在全酶以T7 DNA模板上的AnpppA、CTP、UTP和GTP合成的RNA的5'-末端存在AnpppA残基。
