Denisova L Ia, Zagrebel'nyĭ S N, Kileva E V, Pustoshilova N M, Torbina A I
Mol Biol (Mosk). 1978 Jul-Aug;12(4):766-71.
The behavior of nucleoside triphosphates (NTP) in the RNA-polymerase system from E. coli was studied. The conversion of NTP to the corresponding nucleoside diphosphate (NDP) was observed. This phenomenon was accounted for a contaminating enzyme activity in the RNA-polymerase preparations. The possibility to remove such a contamination was demonstrated, the best technique being the DNA-cellulose affinity chromatography. The purified enzyme does not catalyze the intermediate formation of NDP's from NTP's during RNA synthesis with poly(U) and poly(dG)-poly(dC) as templates.
对大肠杆菌RNA聚合酶系统中三磷酸核苷（NTP）的行为进行了研究。观察到NTP向相应二磷酸核苷（NDP）的转化。这种现象是由RNA聚合酶制剂中的一种污染酶活性引起的。证明了去除这种污染的可能性，最佳技术是DNA-纤维素亲和色谱法。纯化后的酶在以聚（U）和聚（dG）-聚（dC）为模板的RNA合成过程中，不会催化NTP形成NDP中间体。
