Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Egyptian Russian University, Egypt.
Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Egyptian Russian University, Egypt.
Int J Pharm. 2024 Sep 30;663:124541. doi: 10.1016/j.ijpharm.2024.124541. Epub 2024 Jul 30.
Although the use of thermoplastic polyurethane (Tpu) nanofiber mats as wound dressings is of great interest due to their mechanical properties, they are hindered by their poor wettability and bioavailability. In this study, we aimed to improve the cellular affinity of Tpu nanofiber mats for skin disorders by incorporating extracted collagen (Col) from tendons and physically mixed with a layer of phytoceramides (Phyto) to produce TpuCol@X-Phyto mats in which the weight % of Phyto relatively to the weight of the solution was X = 0.5, 1.0, or 1.5 wt% via facile electrospinning approach. The collective observations strongly indicate the successful incorporation and retention of Phyto within the TpuCol architecture. An increase in the Phyto concentration decreased the water contact angle from 69.4° ± 3.47° to 57.9° ± 2.89°, demonstrating improvement in the hydrophilicity of Tpu and binary blend TpuCol nanofiber mats. The mechanical property of 1.0 wt% Phyto aligns with practical requirements owing to the presence of two hydroxyl groups and the amide linkage likely contributing to various hydrogen bonds, providing mechanical strength to the channel structure and a degree of rigidity essential for transmitting mechanical stress. The proliferation of human skin fibroblast (HSF) peaked significantly 100 % with TpuCol@X-Phyto mats coated for X =1.0 and 1.5 wt% of Phyto. Electrospun scaffolds with the highest Phyto content have shown the lowest degree of hemolysis, demonstrating the high level of compatibility between them and blood. The TpuCol@1.5Phyto mat also demonstrated higher efficacy in antibacterial and antioxidant activities, achieving a rate of DPPH radical scavenging of 83.3 % for this latter property. The most notable wound closure among all tested formulations was attributed to higher Phyto. Thus, the developed TpuCol@1.5Phyto nanofiber formula exhibited enhanced healing in an in vitro epidermal model.
尽管热塑性聚氨酯（TPU）纳米纤维垫因其机械性能而在用作伤口敷料方面具有很大的吸引力，但由于其润湿性和生物利用度差而受到阻碍。在这项研究中，我们旨在通过将肌腱中提取的胶原蛋白（Col）掺入其中并与一层植物神经酰胺（Phyto）物理混合，来改善 TPU 纳米纤维垫对皮肤疾病的细胞亲和力，从而生产 TpuCol@X-Phyto 垫，其中 Phyto 的重量相对溶液的重量为 X=0.5、1.0 或 1.5wt%，通过简单的静电纺丝方法。综合观察结果强烈表明 Phyto 成功掺入并保留在 TpuCol 结构中。Phyto 浓度的增加使水接触角从 69.4°±3.47°降低至 57.9°±2.89°，表明 TPU 和二元共混 TpuCol 纳米纤维垫的亲水性得到改善。由于存在两个羟基和酰胺键，1.0wt%Phyto 的机械性能与实际要求一致，这可能有助于形成各种氢键，为通道结构提供机械强度，并提供传递机械应力所需的一定程度的刚性。用 TpuCol@X-Phyto 垫涂覆 X=1.0 和 1.5wt%Phyto 的 HSF 的增殖显著达到峰值 100%。具有最高 Phyto 含量的静电纺丝支架的溶血程度最低，表明它们与血液之间具有高度的相容性。TpuCol@1.5Phyto 垫在抗菌和抗氧化活性方面也表现出更高的功效，在后一种性质下，DPPH 自由基清除率达到 83.3%。所有测试配方中最显著的伤口闭合归因于更高的 Phyto。因此，开发的 TpuCol@1.5Phyto 纳米纤维配方在体外表皮模型中表现出增强的愈合效果。
