Bionano Systems Engineering Department, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
Carbohydr Polym. 2012 Nov 6;90(4):1786-93. doi: 10.1016/j.carbpol.2012.07.071. Epub 2012 Aug 3.
Dextran is a versatile biomacromolecule for preparing electrospun nanofibrous membranes by blending with either water-soluble bioactive agents or hydrophobic biodegradable polymers for biomedical applications. In this study, an antibacterial electrospun scaffold was prepared by electrospinning of a solution composed of dextran, polyurethane (PU) and ciprofloxacin HCl (CipHCl) drug. The obtained nanofiber mats have good morphology. The mats were characterized by various analytical techniques. The interaction parameters between fibroblasts and the PU-dextran and PU-dextran-drug scaffolds such as viability, proliferation, and attachment were investigated. The results indicated that the cells interacted favorably with the scaffolds especially the drug-containing one. Moreover, the composite mat showed good bactericidal activity against both of Gram-positive and Gram-negative bacteria. Overall, our results conclude that the introduced scaffold might be an ideal biomaterial for wound dressing applications.
葡聚糖是一种多功能生物大分子，可与水溶性生物活性剂或疏水性可生物降解聚合物混合，用于制备用于生物医学应用的电纺纳米纤维膜。在这项研究中，通过将葡聚糖、聚氨酯（PU）和盐酸环丙沙星（CipHCl）药物的溶液进行静电纺丝，制备了一种具有抗菌作用的静电纺丝支架。所得纳米纤维垫具有良好的形态。通过各种分析技术对垫子进行了表征。研究了成纤维细胞与 PU-葡聚糖和 PU-葡聚糖-药物支架之间的相互作用参数，如活力、增殖和附着。结果表明，细胞与支架特别是含药支架的相互作用良好。此外，复合垫对革兰氏阳性菌和革兰氏阴性菌均显示出良好的杀菌活性。总的来说，我们的结果表明，所引入的支架可能是一种理想的用于伤口敷料应用的生物材料。
