Department of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey.
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey.
Reprod Domest Anim. 2024 Aug;59(8):e14692. doi: 10.1111/rda.14692.
Anti-Müllerian hormone (AMH) is a hormone produced by growing preantral and antral follicles of the ovary. AMH is accepted as an important biomarker for fertility and superovulation parameters in livestock species. This study aimed to evaluate changes in serum AMH level in the oestrous cycle, repeatability of AMH, the effect of age on serum AMH level and the effects of AMH on litter size in Romanov sheep. In the study, a total of 36 Romanov sheep were used as animal material. First blood samples (0th day) were collected from 36 ewes to evaluate AMH and progesterone levels. Second blood samples were collected randomly from 20 ewes 9 days after first sampling to compare AMH levels at two different periods of the oestrous cycle in Romanov ewes. The ewes were categorized into three groups as low, medium and high AMH based on their first AMH levels. Results indicated that serum AMH level did not change during the oestrous and dioestrous phases of the oestrous cycle and two random time points of the oestrous cycle (p > .05). Pearson correlation analysis revealed that there is a high (r = .95) and significant (p < .001) correlation between AMH levels at the 0th (AMH-1) and 9th (AMH-2) days. The effect of AMH level on litter size was found to be significant. Litter size was significantly higher in the high AMH group than in the low AMH group (p < .05). In addition, the age of ewes did not affect serum AMH levels (p > .05). ROC analysis indicates that AMH cut-off value >320 pg/mL with 70% sensitivity and 100% specificity can be used for litter size in Romanov ewes. In conclusion, AMH is highly repeatable and its serum AMH level did not change during the oestrous cycle in Romanov sheep. In addition, AMH affects litter size and can be reliably used as a marker for litter size in Romanov sheep.
抗缪勒管激素（AMH）是由卵巢内的原始卵泡和窦前卵泡分泌的激素。AMH 被认为是家畜中评估生育力和超排参数的重要生物标志物。本研究旨在评估发情周期中血清 AMH 水平的变化、AMH 的可重复性、年龄对血清 AMH 水平的影响以及 AMH 对罗曼诺夫羊产仔数的影响。在这项研究中，共使用了 36 只罗曼诺夫羊作为动物材料。首先，从 36 只母羊中采集第 0 天的第一份血样，以评估 AMH 和孕酮水平。其次，从 20 只母羊中随机采集第二次血样，采集时间为第一次采样后 9 天，以比较罗曼诺夫母羊发情周期的两个不同时期的 AMH 水平。根据第一次 AMH 水平，将母羊分为低、中、高 AMH 三组。结果表明，血清 AMH 水平在发情和间情期以及发情周期的两个随机时间点都没有变化（p>.05）。皮尔逊相关分析显示，第 0 天（AMH-1）和第 9 天（AMH-2）的 AMH 水平之间存在高度相关（r=95）和显著相关（p<.001）。AMH 水平对产仔数的影响是显著的。高 AMH 组的产仔数明显高于低 AMH 组（p<.05）。此外，母羊的年龄不影响血清 AMH 水平（p>.05）。ROC 分析表明，AMH 截断值>320pg/mL 时，灵敏度为 70%，特异性为 100%，可用于罗曼诺夫羊的产仔数。总之，AMH 高度可重复，在罗曼诺夫羊的发情周期中，其血清 AMH 水平没有变化。此外，AMH 影响产仔数，可作为罗曼诺夫羊产仔数的可靠标志物。
