Wang Shenyuan, Bai Yanyu, Wang Daqing, Zhang Ming, Alatan Suhe, Cang Ming, Jin Hai, Li Changqing, Du Guangchen, Cao Guifang, Tong Bin
Inner Mongolia Key Laboratory of Biomanufacture, College of Life Sciences, Inner Mongolia Agriculture University, Hohhot 010020, China.
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010020, China.
Vet Sci. 2025 Mar 2;12(3):222. doi: 10.3390/vetsci12030222.
Reproductive performance in sheep plays a crucial role in determining the economic efficiency of the industry, with increasing litter size being a key focus for genetic improvement. The gene is widely recognized as a major gene influencing sheep fertility. In this study, specific mutations in the gene of Gobi short tail sheep were identified through direct sequencing, and these mutations were genotyped using the MassARRAY system. The g.54285159_54285161TTA indel was significantly associated with litter size in Gobi short tail sheep ( < 0.05). Three mutations, including g.54291460G>A, g.54288671C>T, and the g.54285159_54285161TTA indel, were significantly associated with litter size in Ujimqin sheep ( < 0.05). Furthermore, the promoter activity analysis demonstrated that the A allele exhibited significantly higher promoter activity compared to the G allele of the g.54291460G>A mutation. These findings highlight valuable genetic markers for improving sheep litter size and provide a robust theoretical foundation for further research on the gene's role in reproduction.
绵羊的繁殖性能在决定该产业的经济效益方面起着至关重要的作用,增加产仔数是遗传改良的关键重点。该基因被广泛认为是影响绵羊繁殖力的一个主要基因。在本研究中,通过直接测序鉴定了戈壁短尾羊该基因的特定突变,并使用MassARRAY系统对这些突变进行基因分型。g.54285159_54285161TTA插入缺失与戈壁短尾羊的产仔数显著相关(<0.05)。三个突变,包括g.54291460G>A、g.54288671C>T和g.54285159_54285161TTA插入缺失,与乌珠穆沁羊的产仔数显著相关(<0.05)。此外,启动子活性分析表明,g.54291460G>A突变的A等位基因与G等位基因相比表现出显著更高的启动子活性。这些发现突出了用于提高绵羊产仔数的有价值的遗传标记,并为进一步研究该基因在繁殖中的作用提供了坚实的理论基础。