Hestiantoro Andon, Noor Al Maghfira Rachellina, Fathmasari Ratna, Rahmala Febri Ririn, Ongko Joyo Ericko, Muharam Raden, Pratama Gita, Bowolaksono Anom
Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo General Hospital, Jakarta, Indonesia.
Cluster of Human Reproduction, Fertility and Family Planning, Indonesia Medical Education and Research Institute, University of Indonesia, Jakarta, Indonesia.
Int J Reprod Biomed. 2024 Jul 8;22(5):395-404. doi: 10.18502/ijrm.v22i5.16440. eCollection 2024 May.
Since kisspeptin () in the hypothalamus is affected by the inhibitory effect of dynorphin, it raises questions about the controlled balance of these 2 neuropeptides in women with and without polycystic ovary syndrome (PCOS).
This study compares the expression levels of , dynorphin, neurokinin-B, leptin, and neuropeptide-Y in women with and without PCOS.
In this cross-sectional study, the peripheral blood samples of 20 women with PCOS and 20 women without PCOS who referred to Yamin Kencana Clinic, Cipto Mangunkusumo hospital, Jakarta, Indonesia were enrolled from August-December 2022. mRNA relative expression of genes related to the central factors associated with PCOS, such as leptin, neuropeptide-Y, , tachykinin-3, and prodynorphin (), in PCOS and non-PCOS populations were examined. Gene quantification was carried out by the quantitative polymerase chain reaction method.
The / ratio was significantly higher in the PCOS group than in the control group (p = 0.02), and the was lower in the PCOS group than the control group (p 0.001). Moreover, the positive correlation between and the / ratio was significantly stronger in the PCOS group than in the control group (R = 0.93; p 0.001 vs. R = 0.66, p 0.001).
Our results suggest that an increased ratio in PCOS women is related to diminished dynorphin expression. Low expression of the gene encoding dynorphin and a high / ratio is highly specific to PCOS.
由于下丘脑的 kisspeptin（）受强啡肽抑制作用的影响，这引发了关于患有和未患有多囊卵巢综合征（PCOS）的女性中这两种神经肽的平衡调控问题。
本研究比较了患有和未患有 PCOS 的女性中 kisspeptin（）、强啡肽、神经激肽 B、瘦素和神经肽 Y 的表达水平。
在这项横断面研究中，于 2022 年 8 月至 12 月招募了 20 名患有 PCOS 的女性和 20 名未患有 PCOS 的女性，她们均前往印度尼西亚雅加达 Cipto Mangunkusumo 医院的 Yamin Kencana 诊所就诊。检测了 PCOS 和非 PCOS 人群中与 PCOS 相关的中枢因子（如瘦素、神经肽 Y、kisspeptin（）、速激肽 -3 和前强啡肽（））相关基因的 mRNA 相对表达。通过定量聚合酶链反应方法进行基因定量。
PCOS 组的 kisspeptin（）/强啡肽比值显著高于对照组（p = 0.02），且 PCOS 组的强啡肽低于对照组（p < 0.001）。此外，PCOS 组中 kisspeptin（）与 kisspeptin（）/强啡肽比值之间的正相关性显著强于对照组（R = 0.93；p < 0.001 对比 R = 0.66，p < 0.001）。
我们的结果表明，PCOS 女性中 kisspeptin（）/强啡肽比值升高与强啡肽表达减少有关。编码强啡肽的基因低表达和高 kisspeptin（）/强啡肽比值对 PCOS 具有高度特异性。
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