Xia Wenqian, Yang Yupeng, Zhang Chenguang, Liu Chunsheng, Xiao Kun, Xiao Xiao, Wu Junkai, Shen Yanhong, Zhang Libin, Su Kai
College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Qinhuangdao, China.
Front Genet. 2024 Jul 18;15:1438276. doi: 10.3389/fgene.2024.1438276. eCollection 2024.
Low temperature pose significant challenges to peach cultivation, causing severe damage to peach buds and restricting production and distribution. Ethylene, an important phytohormone, plays a critical role in enhancing plant cold resistance. Structural genes and transcription factors involved in ethylene biosynthesis and signal transduction pathways are associated with cold resistance. However, no research has specifically addressed their roles in peach cold resistance. In this study, we aimed for cold-resistance gene discovery in cold-sensitive peach cultivar "21Shiji" (21SJ) and cold-resistance cultivar "Shijizhixing" (SJZX) using RNA-seq and gas chromatography. The findings revealed that under cold stress conditions, ethylene biosynthesis in "SJZX" was significantly induced. Subsequently, a structural gene, , involved in ethylene biosynthesis in peach buds was significantly upregulated and showed a higher correlation with ethylene release rate. To identify potential transcription factors associated with expression and ethylene signal transduction, weighted gene co-expression network analysis was conducted using RNA-seq data. Four transcription factors: , , and , were identified. These findings provide valuable theoretical insights for investigating the regulatory mechanisms of peach cold resistance and guiding breeding strategies.
低温给桃树栽培带来了巨大挑战，会对桃芽造成严重损害，并限制产量和销售。乙烯作为一种重要的植物激素，在增强植物抗寒性方面起着关键作用。参与乙烯生物合成和信号转导途径的结构基因和转录因子与抗寒性相关。然而，尚无研究专门探讨它们在桃树抗寒中的作用。在本研究中，我们旨在利用RNA测序和气相色谱法在冷敏感桃品种“21世纪”（21SJ）和抗寒品种“世纪之星”（SJZX）中发现抗寒基因。研究结果表明，在冷胁迫条件下，“SJZX”中的乙烯生物合成显著诱导。随后，一个参与桃芽乙烯生物合成的结构基因被显著上调，并且与乙烯释放速率呈现出更高的相关性。为了鉴定与该基因表达和乙烯信号转导相关的潜在转录因子，利用RNA测序数据进行了加权基因共表达网络分析。鉴定出了四个转录因子：[具体转录因子名称缺失]、[具体转录因子名称缺失]、[具体转录因子名称缺失]和[具体转录因子名称缺失]。这些发现为研究桃树抗寒调控机制和指导育种策略提供了有价值的理论见解。
