State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine, Harbin, China.
State Key Laboratory of Dampness Syndrome, The Second Affiliated Hospital Guangzhou Medical University, Guangzhou, China.
Curr Pharm Des. 2024;30(34):2701-2717. doi: 10.2174/0113816128308622240709102830.
Chemotherapy-induced Peripheral Neuropathy (CIPN) is a common complication that arises from the use of anticancer drugs. Huangqi Guizhi Wuwu Decoction (HGWWD) is an effective classic prescription for treating CIPN; however, the mechanism of the activity is not entirely understood.
This study aimed to investigate the remedial effects and mechanisms of HGWWD on CIPN.
Changes in behavioral, biochemical, histopathological, and biomarker indices were used to evaluate the efficacy of HGWWD treatment. Ultra-high-performance liquid chromatography/mass spectrometry combined with the pattern recognition method was used to screen biomarkers and metabolic pathways related to CIPN. The results of pathway analyses were verified by protein blotting experiments.
A total of 29 potential biomarkers were identified and 13 metabolic pathways were found to be involved in CIPN. In addition HGWWD reversed the levels of 19 biomarkers. Prostaglandin H2 and 17α,21-dihydroxypregnenolone were targeted as core biomarkers.
This study provides scientific evidence to support the finding that HGWWD mainly inhibits the inflammatory response during CIPN by regulating arachidonic acid metabolism.
化疗引起的周围神经病变（CIPN）是一种常见的并发症，源于抗癌药物的使用。黄芪桂枝五物汤（HGWWD）是治疗 CIPN 的有效经典方剂；然而，其作用机制尚不完全清楚。
本研究旨在探讨 HGWWD 治疗 CIPN 的疗效及机制。
采用行为学、生化、组织病理学和生物标志物指标的变化来评估 HGWWD 治疗的效果。超高效液相色谱/质谱联用结合模式识别方法筛选与 CIPN 相关的生物标志物和代谢途径。通过蛋白印迹实验验证通路分析的结果。
共鉴定出 29 个潜在的生物标志物，发现 13 条代谢途径与 CIPN 有关。此外，HGWWD 还逆转了 19 种生物标志物的水平。前列腺素 H2 和 17α,21-二羟孕烯酮被作为核心生物标志物。
本研究为 HGWWD 主要通过调节花生四烯酸代谢抑制 CIPN 期间的炎症反应提供了科学依据。
