Sharma Shagun, Jhalora Vandana, Mathur Shubhita, Bist Renu
Department of Zoology, Centre of Advanced Studies, University of Rajasthan, 302004, Jaipur, India.
Infect Disord Drug Targets. 2025;25(2):e310724232507. doi: 10.2174/0118715265278809240101073539.
A biofilm refers to a community of microbial cells that adhere to surfaces that are surrounded by an extracellular polymeric substance. Bacteria employ various defence mechanisms, including biofilm formation, to enhance their survival and resistance against antibiotics.
The current study aims to investigate the resistance patterns of and in both biofilms and their planktonic forms.
E. coli and B. subtilis were used to compare resistance patterns in biofilms versus planktonic forms of bacteria. An antibiotic disc diffusion test was performed to check the resistance pattern of biofilm and planktonic bacteria against different antibiotics such as penicillin G, streptomycin, and ampicillin. Biofilm formation and its validation were done by using quantitative (microtiter plate assay) and qualitative analysis (Congo red agar media).
A study of surface-association curves of and revealed that surface adhesion in biofilms was continuously constant as compared to their planktonic forms, thereby confirming the increased survival of bacteria in biofilms. Also, biofilms have shown high resistance towards the penicillin G, ampicillin and streptomycin as compared to their planktonic form.
It is safely inferred that E. coli and B. subtilis, in their biofilms, become increasingly resistant to penicillin G, ampicillin and streptomycin.
生物膜是指附着在被细胞外聚合物包围的表面上的微生物细胞群落。细菌采用多种防御机制，包括形成生物膜，以提高其生存能力和对抗生素的耐药性。
本研究旨在调查大肠杆菌和枯草芽孢杆菌在生物膜及其浮游形式中的耐药模式。
使用大肠杆菌和枯草芽孢杆菌比较生物膜形式与浮游形式细菌的耐药模式。进行抗生素纸片扩散试验，以检查生物膜和浮游细菌对不同抗生素（如青霉素G、链霉素和氨苄青霉素）的耐药模式。通过定量（微量滴定板法）和定性分析（刚果红琼脂培养基）进行生物膜形成及其验证。
对大肠杆菌和枯草芽孢杆菌表面结合曲线的研究表明，与浮游形式相比，生物膜中的表面粘附持续恒定，从而证实了生物膜中细菌生存能力的提高。此外，与浮游形式相比，生物膜对青霉素G、氨苄青霉素和链霉素表现出高耐药性。
可以有把握地推断，大肠杆菌和枯草芽孢杆菌的生物膜对青霉素G、氨苄青霉素和链霉素的耐药性越来越强。
