State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Citrus Research Institute, Southwest University & Chinese Academy of Agricultural Sciences, Chongqing 400712, China.
J Agric Food Chem. 2024 Aug 21;72(33):18401-18411. doi: 10.1021/acs.jafc.4c03876. Epub 2024 Aug 2.
The uptake, translocation, and accumulation of mefentrifluconazole (MFZ), an innovative chiral triazole fungicide, in plants at the enantiomeric level are still unclear. Herein, we investigated the patterns and mechanisms of enantiomeric uptake, bioaccumulation, and translocation through several experiments. Rac-MFZ shows the strongest uptake and bioaccumulation capacity in wheat compared with its enantiomers, while S-(+)-MFZ has the highest translocation potential. Molecular docking provided evidence of the stronger translocation ability of S-(+)-MFZ than R-(-)-MFZ. Split-root experiments showed that MFZ and its enantiomers could undergo long-distance transport within the wheat. Active transport or facilitated and simple diffusion may be involved in the wheat uptake of MFZ. The limited acropetal translocation capability of MFZ may be attributed to the dominant uptake pathway of apoplastic. The concentrations of Rac-MFZ in different subcellular fractions varied greatly. In summary, this study provides novel insights for further understanding the behaviors of MFZ and its enantiomers in plants.
麦呋氟菌唑（Mefentrifluconazole，MFZ）是一种新型手性三唑类杀菌剂，其在植物体内的对映体水平上的吸收、转运和积累情况尚不清楚。本研究通过多项实验，研究了其对映体的吸收、生物积累和转运模式及机制。与对映体相比， rac-MFZ 表现出在小麦中最强的吸收和生物积累能力，而 S-(+)-MFZ 具有最高的转运潜力。分子对接为 S-(+)-MFZ 比 R-(-)-MFZ 具有更强的转运能力提供了证据。劈根实验表明，MFZ 及其对映体可以在小麦中进行长距离运输。主动运输或易化扩散和简单扩散可能参与了 MFZ 在小麦中的吸收。MFZ 向地上部的有限转运能力可能归因于质外体的主要吸收途径。不同亚细胞组分中 rac-MFZ 的浓度差异很大。综上所述，本研究为进一步了解 MFZ 及其对映体在植物中的行为提供了新的见解。
