Zhejiang Province Key Laboratory for Food Safety, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China; Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China.
Sci Total Environ. 2023 Dec 20;905:167317. doi: 10.1016/j.scitotenv.2023.167317. Epub 2023 Sep 23.
Mefentrifluconazole (MFZ) is an azole fungicide that is placed in agriculture for the control of fungal hazards. However, due to their non-biodegradability, azole fungicides can accumulate in plants, animals, and the environment, thus becoming a major health concern worldwide. In this study, we exposed 7-week-old C57BL/6 mice to 10, 30, and 100 mg/kg of MFZ for 28 d to assess the toxic effects of MFZ on the liver and gut tissues of the mice. Histopathological, biochemical indexes, and transcriptomic analyses revealed that MFZ exposure disrupted the liver structure and hepatic lipid metabolism as well as damaged gut barrier function and promoted inflammation in mice. Moreover, 16S rRNA sequencing demonstrated that MFZ exposure significantly increased the abundance of patescibacteria at the generic level. Also, MFZ exposure increased the abundance of bacterial genera associated with inhibition of glycolipid metabolism. These results suggested that the disruption of liver lipid metabolism caused by MFZ exposure may be caused by changes in gut microbiota function. This study provided a new disease occurrence study for risk assessment of MFZ and strengthened the focus on some novel fungicides.
美芬净（MFZ）是一种唑类杀菌剂，用于农业防治真菌危害。然而，由于其不可生物降解性，唑类杀菌剂可能在植物、动物和环境中积累，因此成为全球主要的健康关注点。在这项研究中，我们将 7 周龄 C57BL/6 小鼠暴露于 10、30 和 100mg/kg 的 MFZ 中 28 天，以评估 MFZ 对小鼠肝脏和肠道组织的毒性作用。组织病理学、生化指标和转录组分析表明，MFZ 暴露破坏了肝脏结构和肝脂质代谢，损害了肠道屏障功能，并促进了小鼠的炎症。此外，16S rRNA 测序表明，MFZ 暴露显著增加了 patescibacteria 在属水平上的丰度。此外，MFZ 暴露增加了与抑制糖脂代谢相关的细菌属的丰度。这些结果表明，MFZ 暴露引起的肝脂质代谢紊乱可能是由肠道微生物群功能的变化引起的。本研究为 MFZ 的风险评估提供了新的疾病发生研究，并加强了对一些新型杀菌剂的关注。
