Department of Pediatric Pulmonology and Allergy, Hôpital Jeanne de Flandre, CHU Lille, Université de Lille, Lille Cedex, France.
INSERM Unit 1019, CNRS UMR 9017, CHU Lille, Institut Pasteur de Lille, Center for infection and immunity of Lille, Université de Lille, Lille Cedex, France.
J Med Virol. 2024 Aug;96(8):e29804. doi: 10.1002/jmv.29804.
Although rhinoviruses play a major role in exacerbations of childhood asthma, the presence of rhinovirus (RV) RNA in plasma, referred to as viremia, has been investigated in a few studies. The aim of the study was to investigate the presence of rhinovirus viremia at the time of asthma exacerbation and to describe the molecular characteristics of rhinoviruses associated with viremia. We conducted an observational, prospective, multicenter study in eight pediatric hospitals (VIRASTHMA2). Preschool-aged recurrent wheezers (1-5 years) hospitalized for a severe exacerbation were included. Reverse-transcription polymerase chain reaction (RT-PCR) and molecular typing for RV/enteroviruses (EV) were performed on nasal swabs and plasma. Plasma specimens were available for 105 children with positive RT-PCR for RV/EV in respiratory specimens. Thirty-six (34.3%) had positive viremia. In plasma, 28 (82.4%) of the typable specimens were RV-C, five (14.7%) were EV-D68, and one was RV-A (2.9%). In all cases, the RV/EV type was identical in the plasma and respiratory specimens. In conclusion, RV/EV viremia is frequent in severe exacerbations of preschool recurrent wheezers, particularly in RV-C infections.
虽然鼻病毒在儿童哮喘恶化中起主要作用，但已有少数研究调查了血浆中鼻病毒 (RV) RNA 的存在，即病毒血症。本研究旨在调查哮喘恶化时鼻病毒血症的存在，并描述与病毒血症相关的鼻病毒的分子特征。我们在八家儿童医院进行了一项观察性、前瞻性、多中心研究（VIRASTHMA2）。纳入因严重恶化而住院的 1-5 岁反复喘息的学龄前儿童。对鼻拭子和血浆进行逆转录聚合酶链反应 (RT-PCR) 和 RV/肠道病毒 (EV) 的分子分型。呼吸道标本 RT-PCR 检测 RV/EV 阳性的 105 例患儿有血浆标本。36 例（34.3%）存在病毒血症阳性。在可分型的标本中，血浆中 28 例（82.4%）为 RV-C，5 例（14.7%）为 EV-D68，1 例为 RV-A（2.9%）。在所有情况下，血浆和呼吸道标本中的 RV/EV 型均相同。总之，鼻病毒/肠道病毒病毒血症在学龄前反复喘息严重恶化中很常见，尤其是在 RV-C 感染中。
