Stomatology Department, Ma'anshan People's Hospital, Ma'anshan, Anhui, China.
Bengbu Medical University, Bengbu, Anhui, China.
Technol Health Care. 2024;32(6):4695-4705. doi: 10.3233/THC-240930.
Oral cancer has a high worldwide incidence and mortality rate showing an upward trend year by year, predominantly occurring in emerging countries. Oral squamous cell carcinoma (OSCC) is one of the main types of oral cancer, accounting for more than 90% of all cases in oral cancer.
To evaluate the diagnostic value of 8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-iso-Prostaglandin F2alpha (8-iso-PGF2α) and tumor necrosis factor (TNF)-α as biomarkers in the early carcinogenesis of erosive oral lichen planus (EOLP) by measuring their levels in the blood of patients with EOLP and oral squamous cell carcinoma (OSCC).
A total of 69 patients were enrolled in this case-control study [including an OSCC group (n= 23), an EOLP group (n= 23), and an age- and gender-matched healthy control group (n= 23)]. Blood levels of 8-OHdG, 8-iso-PGF2α and TNF-α were measured using enzyme-linked immunosorbent assay (ELISA). Statistical differences in these indicators among the three groups were analyzed.
Plasma levels of 8-OHdG and 8-iso-PGF2α in the OSCC group were significantly higher than those in both the EOLP group and the control group (all P< 0.05); no significant statistical difference was found between the EOLP group and the control group. Serum levels of TNF-α in both the OSCC and EOLP groups were elevated compared with the control group, showing significant differences among all three groups (all P< 0.05). Receiver operating characteristic (ROC) curves revealed that plasma 8-OHdG and 8-iso-PGF2α levels and serum TNF-α levels had diagnostic effects on early carcinogenesis in EOLP patients. When these indicators were combined for diagnosis, the diagnostic effect was enhanced, with an area under the ROC curve (AUC) values of 0.819.
8-OHdG, 8-iso-PGF2α and TNF-α may serve as biological indicators for monitoring the early carcinogenesis of EOLP, and the diagnostic effect was augmented when these indicators were combined.
口腔癌在全球的发病率和死亡率都很高，且呈逐年上升趋势，主要发生在新兴国家。口腔鳞状细胞癌（OSCC）是口腔癌的主要类型之一，占口腔癌所有病例的 90%以上。
通过检测糜烂型口腔扁平苔藓（EOLP）患者和口腔鳞状细胞癌（OSCC）患者血液中 8-羟基-2'-脱氧鸟苷（8-OHdG）、8-异前列腺素 F2α（8-iso-PGF2α）和肿瘤坏死因子（TNF）-α的水平，评估其作为 EOLP 早期癌变生物标志物的诊断价值。
本病例对照研究纳入了 69 名患者[包括 OSCC 组（n=23）、EOLP 组（n=23）和年龄、性别匹配的健康对照组（n=23）]。采用酶联免疫吸附试验（ELISA）检测 8-OHdG、8-iso-PGF2α 和 TNF-α的血液水平。分析三组间这些指标的差异。
OSCC 组血浆 8-OHdG 和 8-iso-PGF2α 水平明显高于 EOLP 组和对照组（均 P<0.05）；EOLP 组与对照组之间无显著统计学差异。OSCC 组和 EOLP 组血清 TNF-α水平均高于对照组，三组间差异有统计学意义（均 P<0.05）。受试者工作特征（ROC）曲线显示，血浆 8-OHdG 和 8-iso-PGF2α 水平及血清 TNF-α水平对 EOLP 患者早期癌变具有诊断作用。当这些指标联合诊断时，诊断效果增强，ROC 曲线下面积（AUC）值为 0.819。
8-OHdG、8-iso-PGF2α 和 TNF-α可能作为监测 EOLP 早期癌变的生物学指标，联合这些指标可提高诊断效果。
