Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, the Netherlands; Kavli Institute of Nanoscience, Delft, the Netherlands.
Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, the Netherlands.
Cell Host Microbe. 2024 Aug 14;32(8):1427-1443.e8. doi: 10.1016/j.chom.2024.07.007. Epub 2024 Aug 1.
Prokaryotes have evolved a multitude of defense systems to protect against phage predation. Some of these resemble eukaryotic genes involved in antiviral responses. Here, we set out to systematically project the current knowledge of eukaryotic-like antiviral defense systems onto prokaryotic genomes, using Pseudomonas aeruginosa as a model organism. Searching for phage defense systems related to innate antiviral genes from vertebrates and plants, we uncovered over 450 candidates. We validated six of these phage defense systems, including factors preventing viral attachment, R-loop-acting enzymes, the inflammasome, ubiquitin pathway, and pathogen recognition signaling. Collectively, these defense systems support the concept of deep evolutionary links and shared antiviral mechanisms between prokaryotes and eukaryotes.
原核生物已经进化出多种防御系统来抵御噬菌体的捕食。其中一些防御系统类似于参与抗病毒反应的真核生物基因。在这里，我们旨在使用铜绿假单胞菌作为模式生物，将当前关于真核生物样抗病毒防御系统的知识系统地投射到原核生物基因组上。通过搜索与脊椎动物和植物中的先天抗病毒基因相关的噬菌体防御系统，我们发现了超过 450 个候选基因。我们验证了其中的 6 个噬菌体防御系统，包括阻止病毒附着的因子、R 环作用酶、炎症小体、泛素途径和病原体识别信号。总的来说，这些防御系统支持原核生物和真核生物之间存在深层进化联系和共享抗病毒机制的概念。
