Department of Odonto-Stomatology, Can Tho Eye and Odonto-Stomatology Hospital, Can Tho, 900000, Vietnam.
School of Biomedical Engineering, International University, HCMC 700000, Vietnam.
Biomed Mater. 2024 Aug 13;19(5). doi: 10.1088/1748-605X/ad6ac5.
The design of three-dimensional (3D) scaffolds should focus on creating highly porous, 3D structures with an interconnected pore network that supports cell growth. The scaffold's pore interconnectivity is directly linked to vascularization, cell seeding, guided cell migration, and transportation of nutrients and metabolic waste. In this study, different types of food flavors including monosodium glutamate, sugar, and sodium chloride were used as the porogens along with PCL/PVP blend polymer for solvent casting/particulate leaching method. The morphology, porosity, interconnectivity, chemical composition, water absorption, and mechanical properties of the fabricated scaffolds are carefully characterized. The scaffolds are biocompatible in bothandexperiments and do not trigger any inflammatory response while enhancing new bone formation and vascularization in rabbit calvaria critical-sized defects. The new bone merges and becomes denser along with the experiment timeline. The results indicate that the 3D PCL/PVP scaffolds, using monosodium glutamate as porogen, exhibited suitable biological performance and held promise for bone tissue engineering in oral and maxillofacial surgery.
三维（3D）支架的设计应侧重于创建具有高度多孔性、3D 结构和相互连通的孔网络的支架，以支持细胞生长。支架的孔连通性与血管生成、细胞接种、引导细胞迁移以及营养物质和代谢废物的运输直接相关。在这项研究中，使用不同类型的食品香料，包括谷氨酸钠、糖和氯化钠，与 PCL/PVP 共混聚合物一起通过溶剂浇铸/颗粒沥滤法作为致孔剂。仔细表征了制备的支架的形态、孔隙率、连通性、化学成分、吸水率和机械性能。支架在实验和实验中均具有生物相容性，不会引发炎症反应，同时在兔颅骨临界缺损中增强新骨形成和血管生成。新骨随着实验时间的推移而融合并变得更加密集。结果表明，使用谷氨酸钠作为致孔剂的 3D PCL/PVP 支架表现出合适的生物学性能，有望用于口腔颌面外科的骨组织工程。
