School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Traditional Chinese Medicine Glycomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan.
School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135483. doi: 10.1016/j.ijbiomac.2024.135483. Epub 2024 Sep 10.
Sulfated polysaccharides (SPSs) have excellent physicochemical properties, attracting research interest in the pharmaceutical industry. A previous study extracted SPS (named Suc40) from the edible fungus, Poria cocos and demonstrated that it exhibited anti-inflammatory and anticancer activities. In this study, three fractions of Suc40, Suc40 F1, Suc40 F2, and Suc40 F3, with different molecular weights and sulfate contents were prepared through gel-filtration column chromatography. The molecular weights of F1, F2, and F3 were approximately 616.23, 82.57, and 6.21 kDa, respectively, and their sulfate content were 0.23, 1.65, and 1.90 mmol/g, respectively. The fractions' anti-inflammatory activities were determined by assessing their ability to suppress inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Suc40 F2 and Suc40 F3 suppressed interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production by 60 % and 35 %, respectively. Suc40 F2 and Suc40 F3 suppressed protein kinase B (AKT)/p38 and p38 signaling, which resulted in anti-inflammatory effects. The fractions' anti-lung cancer activity was evaluated by assessing their H1975 cell proliferation inhibition. Suc40 F3 at a concentration of 800 μg/ml exhibited maximal cell proliferation inhibition. The low molecular weight and high sulfate content of Suc40 F3 were associated with its enhanced anti-inflammatory and anti-lung cancer activities.
硫酸化多糖（SPSs）具有优异的物理化学性质，引起了制药行业的研究兴趣。先前的研究从食用真菌茯苓中提取了 SPS（命名为 Suc40），并证明其具有抗炎和抗癌活性。在这项研究中，通过凝胶过滤柱色谱法制备了三种 Suc40 级分，Suc40 F1、Suc40 F2 和 Suc40 F3，它们具有不同的分子量和硫酸基含量。F1、F2 和 F3 的分子量分别约为 616.23、82.57 和 6.21 kDa，硫酸基含量分别为 0.23、1.65 和 1.90 mmol/g。通过评估它们抑制脂多糖（LPS）刺激的 RAW264.7 细胞中炎症细胞因子的能力来确定级分的抗炎活性。Suc40 F2 和 Suc40 F3 分别抑制白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生达 60%和 35%。Suc40 F2 和 Suc40 F3 抑制蛋白激酶 B（AKT）/p38 和 p38 信号通路，从而产生抗炎作用。通过评估它们对 H1975 细胞增殖的抑制作用来评估级分的抗肺癌活性。Suc40 F3 在 800μg/ml 的浓度下表现出最大的细胞增殖抑制作用。Suc40 F3 的低分子量和高硫酸基含量与其增强的抗炎和抗肺癌活性有关。
