State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Department of Cardiology, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
J Environ Sci (China). 2025 Feb;148:107-115. doi: 10.1016/j.jes.2024.01.010. Epub 2024 Jan 18.
The evaluation of toxicity related to polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) is crucial for a comprehensive risk assessment in real-world exposure scenarios. This study employed a controlled feeding experiment to investigate the metabolic effects of dioxin-like compounds (DLCs) on laying hens via feed exposure. Diets enriched with two concentrations (1.17 and 5.13 pg toxic equivalents (TEQ)/g dry weight (dw)) were administered over 14 days, followed by 28 days of clean feed. Metabolomics analyses of blood samples revealed significant metabolic variations between PCDD/Fs and DL-PCBs exposed groups and controls, reflecting the induced metabolic disruption. Distinct changes were observed in sphingosine, palmitoleic acid, linoleate, linolenic acid, taurocholic acid, indole acrylic acid, and dibutyl phthalate levels, implying possible connections between PCDD/Fs and DL-PCBs toxic effects and energy-neuronal imbalances, along with lipid accumulation and anomalous amino acid metabolism, impacting taurine metabolism. Moreover, we identified three differential endogenous metabolites-L-tryptophan, indole-3-acetaldehyde, and indole acrylic acid-as potential ligands for the aryl hydrocarbon receptor (AhR), suggesting their role in mediating PCDD/Fs and DL-PCBs toxicity. This comprehensive investigation provides novel insights into the metabolic alterations induced by PCDD/Fs and DL-PCBs in laying hens, thereby enhancing our ability to assess risks associated with their exposure in human populations.
多氯二苯并对二恶英和呋喃（PCDD/Fs）和类似二恶英的多氯联苯（DL-PCBs）毒性的评估对于真实暴露场景中的综合风险评估至关重要。本研究通过饲料暴露，采用控制喂养实验研究了二恶英样化合物（DLCs）对产蛋鸡的代谢影响。用两种浓度（1.17 和 5.13 pg 毒性当量（TEQ）/克干重（dw））富集的饮食喂养了 14 天，然后用清洁饲料喂养了 28 天。血液样本的代谢组学分析表明，暴露于 PCDD/Fs 和 DL-PCBs 的组与对照组之间存在显著的代谢差异，反映了诱导的代谢紊乱。在神经氨酸、棕榈油酸、亚油酸、亚麻酸、牛胆酸、吲哚丙烯酸和邻苯二甲酸二丁酯水平上观察到明显的变化，表明 PCDD/Fs 和 DL-PCBs 毒性效应与能量-神经元失衡、脂质积累和异常氨基酸代谢之间可能存在联系，影响牛磺酸代谢。此外，我们鉴定了三种差异内源性代谢物——L-色氨酸、吲哚-3-乙醛和吲哚丙烯酸——作为芳烃受体（AhR）的潜在配体，表明它们在介导 PCDD/Fs 和 DL-PCBs 毒性中的作用。这项全面的研究提供了关于 PCDD/Fs 和 DL-PCBs 在产蛋鸡中诱导的代谢变化的新见解，从而提高了我们评估人群暴露相关风险的能力。
