H3K36 甲基转移酶 GhKMT3;1a 和 GhKMT3;2a 促进陆地棉开花。

H3K36 methyltransferase GhKMT3;1a and GhKMT3;2a promote flowering in upland cotton.

机构信息

College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.

出版信息

BMC Plant Biol. 2024 Aug 2;24(1):739. doi: 10.1186/s12870-024-05457-y.

DOI:10.1186/s12870-024-05457-y

PMID:39095699

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11295449/

Abstract

BACKGROUND

The SET domain group (SDG) genes encode histone lysine methyltransferases, which regulate gene transcription by altering chromatin structure and play pivotal roles in plant flowering determination. However, few studies have investigated their role in the regulation of flowering in upland cotton.

RESULTS

A total of 86 SDG genes were identified through genome-wide analysis in upland cotton (Gossypium hirsutum). These genes were unevenly distributed across 25 chromosomes. Cluster analysis revealed that the 86 GhSDGs were divided into seven main branches. RNA-seq data and qRT‒PCR analysis revealed that lysine methyltransferase 3 (KMT3) genes were expressed at high levels in stamens, pistils and other floral organs. Using virus-induced gene silencing (VIGS), functional characterization of GhKMT3;1a and GhKMT3;2a revealed that, compared with those of the controls, the GhKMT3;1a- and GhKMT3;2a-silenced plants exhibited later budding and flowering and lower plant heightwere shorter. In addition, the expression of flowering-related genes (GhAP1, GhSOC1 and GhFT) significantly decreased and the expression level of GhSVP significantly increased in the GhKMT3;1a- and GhKMT3;2a-silenced plants compared with the control plants.

CONCLUSION

A total of 86 SDG genes were identified in upland cotton, among which GhKMT3;1a and GhKMT3;2a might regulate flowering by affecting the expression of GhAP1, GhSOC1, GhFT and GhSVP. These findings will provide genetic resources for advanced molecular breeding in the future.

摘要

背景

SET 结构域组（SDG）基因编码组蛋白赖氨酸甲基转移酶，通过改变染色质结构来调节基因转录，在植物开花决定中发挥关键作用。然而，很少有研究调查它们在陆地棉开花调控中的作用。

结果

通过对陆地棉（Gossypium hirsutum）的全基因组分析，共鉴定出 86 个 SDG 基因。这些基因在 25 条染色体上不均匀分布。聚类分析表明，86 个 GhSDGs 分为七个主要分支。RNA-seq 数据和 qRT-PCR 分析显示，赖氨酸甲基转移酶 3（KMT3）基因在雄蕊、雌蕊和其他花器官中表达水平较高。利用病毒诱导的基因沉默（VIGS），GhKMT3;1a 和 GhKMT3;2a 的功能表征表明，与对照相比，GhKMT3;1a 和 GhKMT3;2a 沉默植株的萌芽和开花时间较晚，株高较矮。此外，与对照植株相比，GhKMT3;1a 和 GhKMT3;2a 沉默植株中开花相关基因（GhAP1、GhSOC1 和 GhFT）的表达显著降低，而 GhSVP 的表达水平显著升高。