Mitov B
Vet Med Nauki. 1985;22(9):45-52.
A method was worked out for the extraction of a cell monolayer from test tubes with the use of 10 per cent acetone solution of celloidin. This made it possible to carry out parallel cytologic and immunofluorescent studies of cultures infected with various viruses. In contrast to the alcohol-ether celloidin used in histology the use of the acetone solution of celloidin, suggested by us, preserved to a larger extent the viruses of different structure and composition, and, therefore, was shown to be more suitable in immunofluorescent investigations. The method was employed for the cytologic and the immunofluorescent identification of newly isolated virus agents. It was also used in the controlled replication of noncytopathogenic and slightly cytopathogenic strains of bovine rotaviruses, corona viruses, respiratory-syncytial virus, pestivirus, and others that required roller cultivation. The celloidin method makes it possible to obtain from one to four preparations with material of the cell monolayer of each test tube, and renders it unnecessary to maintain permanently cultures on glass lamellas.
已制定出一种方法，可使用10%的火棉胶丙酮溶液从试管中提取细胞单层。这使得对感染各种病毒的培养物进行平行的细胞学和免疫荧光研究成为可能。与组织学中使用的酒精 - 乙醚火棉胶不同，我们建议使用的火棉胶丙酮溶液在更大程度上保存了不同结构和组成的病毒，因此，在免疫荧光研究中显示出更适用。该方法用于新分离病毒制剂的细胞学和免疫荧光鉴定。它还用于牛轮状病毒、冠状病毒、呼吸道合胞病毒、瘟病毒等非细胞病变和轻微细胞病变菌株的受控复制，这些病毒需要滚瓶培养。火棉胶法使得从每个试管的细胞单层材料中获得一到四份制剂成为可能，并且无需在玻璃片上永久维持培养物。
