Marshall R G, Gillette K G
Am J Vet Res. 1983 Jul;44(7):1366-9.
Bovine syncytial virus was isolated from mesenteric lymph nodes of young dairy calves, beef cattle, and mature dairy cattle. Isolations were made in cocultures of lymph node specimens and bovine embryonic lung cells or by subpassage of cocultures in which cytopathic effects were not initially detected. The bovine syncytial viral isolates after storage at -65 C for 3 months in either infected cell cultures of supernate fluids from suspensions or sonically treated infected cell monolayers were usually recovered. Bovine syncytial virus also was recovered from cell cultures after storage for 6 months at -25 C.
牛合胞体病毒是从幼年奶牛、肉牛和成年奶牛的肠系膜淋巴结中分离出来的。通过淋巴结标本与牛胚胎肺细胞的共培养，或通过对最初未检测到细胞病变效应的共培养物进行传代培养来进行分离。牛合胞体病毒分离株在 -65°C 下于感染细胞培养物中的悬浮液上清液或经超声处理的感染细胞单层中储存 3 个月后通常能够复苏。牛合胞体病毒在 -25°C 下储存 6 个月后也能从细胞培养物中复苏。
