Schmidt G, Wigand R
Zentralbl Bakteriol A. 1980 Aug;247(3):283-9.
Two procedures to improve virus cultivation from clinical material were evaluated: an intensified adsorption with 0.2 ml inoculum and 20 h rocking in a Bellco rocker and a low-speed centrifugation of the material onto the cultured cells. Prototype strains from 5 virus families (adenoviruses, herpes simplex virus, vaccinia virus, enteroviruses, parainfluenza 2) as well as original specimens from patients (adenovirus, herpesvirus, enterovirus) were studied by endpoint titration in comparison with the standard procedure. In adenoviruses, a quantitative immunofluorescence was performed too. In endpoint titrations, the centrifugation method did almost never lead to an increased virus titer, as compared with the standard method. However, in the immunofluoresence evaluation of adenoviruses the values attained were 3- to 4-fold higher. On the other hand, the intensified adsorption method led to an increased sensitivity in most adenovirus titrations with 4- to 25-fold titer increment, with prototype and original material. The procedure was ineffective in all other viruses studied.
一种是使用0.2毫升接种物进行强化吸附，并在Bellco摇床上摇晃20小时；另一种是将材料低速离心到培养细胞上。通过终点滴定法，将来自5个病毒家族（腺病毒、单纯疱疹病毒、痘苗病毒、肠道病毒、副流感病毒2型）的原型毒株以及患者的原始标本（腺病毒、疱疹病毒、肠道病毒）与标准方法进行比较研究。对于腺病毒，还进行了定量免疫荧光检测。在终点滴定中，与标准方法相比，离心法几乎从未使病毒滴度增加。然而，在腺病毒的免疫荧光评估中，所获得的值高出3至4倍。另一方面，强化吸附法在大多数腺病毒滴定中提高了灵敏度，原型毒株和原始材料的滴度增加了4至25倍。该方法在所有其他研究的病毒中均无效。
