Engineering Technology Center of Department of Education of Anhui Province, College of Chemistry and Materials Engineering, Chaohu University, Chaohu 238024, PR China.
School of Biological and Environmental Engineering, Chaohu University, Chaohu 238024, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 15;323:124922. doi: 10.1016/j.saa.2024.124922. Epub 2024 Jul 31.
It has been well established that Hydrogen sulfide (HS) is involved in various pathophysiological processes. Therefore, accurate monitoring HS levels in vitro or vivo is of great significance in biological systems. Herein, we firstly developed a thiomaleimide-based compound MAL-1 bearing aggregation-induced emission characteristic for selective response toward HS due to its nucleophilicity. The proposed sensor presented prominent sensitivity and selectivity with low detection limit of 75 nM and pseudo-first-order reaction rate constant of 9.65 × 10 s, as well as low cytotoxicity which works well in recognizing HS in real samples and visualizing HS in living cells. Thus, it could be concluded that the novel thiomaleimide-based probe would be a promising tool for assessing intracellular HS levels.
已经证实，硫化氢（HS）参与了各种病理生理过程。因此，在生物系统中准确监测 HS 水平具有重要意义。在此，我们首次开发了一种基于巯基马来酰亚胺的化合物 MAL-1，由于其亲核性，该化合物具有聚集诱导发射特性，可选择性地响应 HS。由于其亲核性，所提出的传感器具有出色的灵敏度和选择性，检测限低至 75 nM，准一级反应速率常数为 9.65×10 s，并且细胞毒性低，可以很好地识别实际样品中的 HS 并在活细胞中可视化 HS。因此，可以得出结论，新型基于硫代马来酰亚胺的探针将是评估细胞内 HS 水平的有前途的工具。
