Senior Resident, Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital, Hyderabad, Telangana State, India.
Professor and Head, Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital, Hyderabad, Telangana State, India.
J Indian Soc Pedod Prev Dent. 2020 Oct-Dec;38(4):367-373. doi: 10.4103/JISPPD.JISPPD_322_20.
Glass ionomer cement (GIC) is known for its antimicrobial activity due to its low pH and fluoride release. The fluoride released has an inhibitory effect on a finite number of bacteria which leads to the risk of recurrent caries. Additives such as chlorhexidine (CHX) and triclosan have been tried to maximize the antibacterial activity of GIC. Although CHX is known for its impressive antimicrobial action, it has adverse after effects which include alteration of commensal oral flora, staining of teeth, etc., Hence, there is a need for a material with improved antimicrobial efficacy with nominal side effects.
The aim of this study is to assess the antimicrobial efficacy of conventional GIC added with Propolis, Chitosan (CH), and CHX against Streptococcus mutans and Lactobacillus acidophilus.
Eighty discs of size 10 × 2 mm are prepared with Conventional GIC and GIC added with Propolis, CH and CHX (n = 10) and tested against S. mutans and L. acidophilus using the agar diffusion assay. Zones of inhibition are measured for day 1, 7, and 14, and the data were tabulated and analyzed.
One-way ANOVA test for intragroup and Tukey's post hoc test for intergroup comparison.
The mean value of zone of inhibition (in mm) against S. mutans on day 14 for Group I, II, III, and IV are 11.70 ± 1.49, 16.50 ± 2.23, 19.30 ± 2.87, and 15.60 ± 2.76, respectively. For L. acidophilus, the mean value of the zone of inhibition (in mm) on day 14 are 8.40 ± 0.97, 9.70 ± 0.68, 16.20 ± 2.04, and 12.50 ± 0.97 for Group I, II, III, and IV, respectively.
Higher antimicrobial activity was shown by GIC with CHX against both strains. GIC with Propolis and GIC with CH were effective in inhibiting S. mutans and L. acidophilus, respectively.
玻璃离子水门汀（GIC）因其低 pH 值和氟释放而具有抗菌活性。释放的氟对有限数量的细菌具有抑制作用，从而导致龋齿复发的风险。已经尝试了添加物，如洗必泰（CHX）和三氯生，以最大限度地提高 GIC 的抗菌活性。尽管 CHX 以其令人印象深刻的抗菌作用而闻名，但它具有不良的副作用，包括改变共生口腔菌群、牙齿染色等，因此，需要一种具有改善的抗菌功效和最小副作用的材料。
本研究旨在评估常规 GIC 中添加蜂胶、壳聚糖（CH）和 CHX 对变形链球菌和嗜酸乳杆菌的抗菌效果。
用常规 GIC 和添加蜂胶、CH 和 CHX 的 GIC 制备大小为 10×2mm 的 80 个圆盘（n=10），并用琼脂扩散法检测对 S. mutans 和 L. acidophilus 的抑菌效果。测量第 1、7 和 14 天的抑菌环直径，并将数据制表和分析。
采用单因素方差分析进行组内比较，采用 Tukey 事后检验进行组间比较。
第 14 天对 S. mutans 的抑菌环直径（mm）的平均值，组 I、II、III 和 IV 分别为 11.70±1.49、16.50±2.23、19.30±2.87 和 15.60±2.76。对于 L. acidophilus，第 14 天的抑菌环直径（mm）的平均值分别为 8.40±0.97、9.70±0.68、16.20±2.04 和 12.50±0.97，分别为组 I、II、III 和 IV。
GIC 与 CHX 对两种菌株均显示出更高的抗菌活性。含蜂胶的 GIC 和含 CH 的 GIC 分别有效抑制 S. mutans 和 L. acidophilus。
