Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, Nanchong, 637000, Sichuan, China; College of Life Sciences, China West Normal University, Nanchong, 637000, Sichuan, China.
Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, Nanchong, 637000, Sichuan, China; College of Life Sciences, China West Normal University, Nanchong, 637000, Sichuan, China; Nanchong Key Laboratory of Wildlife Nutritional Ecology and Disease Prevention and Control, Nanchong, 637000, Sichuan, China.
J Therm Biol. 2024 Jul;123:103935. doi: 10.1016/j.jtherbio.2024.103935. Epub 2024 Aug 2.
Climate change is an increasing concern of stakeholders worldwide. The intestine is severely impacted by the heat stress. This study aimed to investigate the alleviating effects of methionine on the intestinal damage induced by heat stress in mice. The mice were divided into four groups: control group (C), methionine deficiency group (MD), methionine + heat stress group (MH), and methionine deficiency + heat stress group (MDH). Histopathological techniques, PAS-Alcian blue staining, immunohistochemistry method, biochemical quantification method, ELISA, and micro method were used to study the changes in the intestinal mucosal morphology, the number of goblet cells, the expression of tight junction proteins, the peroxide product contents and antioxidant enzyme activities, the intestinal mucosal damage, the content of immunoglobulins and HSP70, the activity of Na/K-ATPase. The results showed that methionine can improve intestinal mucosal morphology (increase the villi height, V/C value, and muscle layer thickness, decrease crypt depth), increase the expression of tight junction proteins (Claudin-1, Occludin, ZO-1) and the content of DAO, decrease the content of intestinal mucosa damage markers (ET, FABP2) and peroxidation products (MDA), increase the activity of antioxidant enzymes (GR, GSH-Px, SOD), the number of goblet cells, the contents of immunoglobulins (sIgA, IgA, IgG, IgM) and stress protein (HSP70), and the activity of Na/K-ATPase. It is suggested that methionine can alleviate intestinal damage in heat-stressed mice.
气候变化是全球利益相关者日益关注的问题。肠道受到热应激的严重影响。本研究旨在探讨蛋氨酸对热应激诱导的小鼠肠道损伤的缓解作用。将小鼠分为四组：对照组（C）、蛋氨酸缺乏组（MD）、蛋氨酸+热应激组（MH）和蛋氨酸缺乏+热应激组（MDH）。采用组织病理学技术、PAS-阿利辛蓝染色、免疫组织化学方法、生化定量方法、ELISA 和微量方法研究了肠黏膜形态、杯状细胞数量、紧密连接蛋白表达、过氧化物产物含量和抗氧化酶活性、肠黏膜损伤、免疫球蛋白和 HSP70 含量、Na/K-ATP 酶活性的变化。结果表明，蛋氨酸能改善肠黏膜形态（增加绒毛高度、V/C 值和肌层厚度，减少隐窝深度），增加紧密连接蛋白（Claudin-1、Occludin、ZO-1）和二胺氧化酶（DAO）的表达，降低肠黏膜损伤标志物（内皮素、脂肪酸结合蛋白 2）和过氧化产物（丙二醛）的含量，提高抗氧化酶（GR、GSH-Px、SOD）、杯状细胞数量、免疫球蛋白（sIgA、IgA、IgG、IgM）和应激蛋白（HSP70）的含量，以及 Na/K-ATP 酶的活性。提示蛋氨酸能缓解热应激小鼠的肠道损伤。
